Martín-Oar J E, Peces R, Ramos B, López-Novoa J M, Hernando L
Endocrinology. 1981 Jun;108(6):2283-6. doi: 10.1210/endo-108-6-2283.
Experiments have been carried out in order to clarify to what extent the absence of PRL renal catabolism in experimental renal insufficiency is responsible for the high PRL circulating levels. Furthermore, the relative contribution of the glomerular filtration rate and peritubular degradation to PRL renal clearance have been assessed. Circulating PRL basal levels were measured by RIA in sham-operated and intact control rats and in three uremic rat models: urine autoinfusion, bilateral ureteral ligation, and bilateral nephrectomy. Plasma PRL basal levels (nanograms per ml; mean +/- SEM) were increased in sham-operated rats (30.3 +/- 5.1) with respect to control animals (18.5 +/- 2.7; P less than 0.05). Bilaterally nephrectomized animals (66.4 +/- 16.4) and those with bilateral ureteral ligation (69.3 +/- 15.9) developed similar hyperprolactinemia, in contrast to urine-autoinfused rats (20.2 +/- 2.1; P less than 0.005) whose hormone levels were similar to those of control animals. Creatinine levels were markedly elevated and comparable in the three uremic rat groups. The results suggest that: 1) hyperprolactinemia in rats in acute renal insufficiency is due primarily to reduced renal function; 2) PRL renal catabolism in the rat requires a certain rate of glomerular filtration; and 3) PRL peritubular degradation does not seem to be relevant in PRL catabolism by rat kidney.
已开展实验，以阐明实验性肾功能不全时PRL肾分解代谢的缺失在多大程度上导致了PRL循环水平升高。此外，还评估了肾小球滤过率和肾小管周围降解对PRL肾清除率的相对贡献。通过放射免疫分析法（RIA）测量了假手术组和完整对照组大鼠以及三种尿毒症大鼠模型（尿液自体输注、双侧输尿管结扎和双侧肾切除）的循环PRL基础水平。与对照动物（18.5±2.7；P<0.05）相比，假手术组大鼠（30.3±5.1）的血浆PRL基础水平（纳克/毫升；平均值±标准误）升高。双侧肾切除的动物（66.4±16.4）和双侧输尿管结扎的动物（69.3±15.9）出现了类似的高催乳素血症，而尿液自体输注的大鼠（20.2±2.1；P<0.005）其激素水平与对照动物相似。三种尿毒症大鼠组的肌酐水平均显著升高且相当。结果表明：1）急性肾功能不全大鼠的高催乳素血症主要是由于肾功能降低；2）大鼠PRL的肾分解代谢需要一定的肾小球滤过率；3）PRL的肾小管周围降解似乎与大鼠肾脏对PRL的分解代谢无关。
