Soboll S, Heldt H W, Scholz R
Hoppe Seylers Z Physiol Chem. 1981 Mar;362(3):247-60. doi: 10.1515/bchm2.1981.362.1.247.
The subcellular distribution of metabolites involved in the transfer of reducing equivalents across the mitochondrial membrane was studied in perfused livers from fed rats. A tenfold increase in the flux rate of the malate-aspartate shuttle and the inhibition of the citrate cycle due to ethanol oxidation, were reflected by characteristic changes in the cytosolic and mitochondrial concentrations of malate, 2-oxoglutarate, aspartate, glutamate and citrate. The data suggest that the malate-aspartate shuttle is triggered by a decrease in the cytosolic oxaloacetate concentration which, due to the cytosolic aspartate aminotransferase equilibrium, leads to an increased efflux of 2-oxoglutarate and aspartate from the mitochondria in exchange for malate and glutamate, respectively. The first site at which the citrate cycle is inhibited appears to be the level of 2-oxoglutarate oxidation.
在喂食大鼠的灌注肝脏中，研究了参与还原当量跨线粒体膜转移的代谢物的亚细胞分布。苹果酸-天冬氨酸穿梭通量率增加了10倍，以及由于乙醇氧化导致柠檬酸循环受到抑制，这些都反映在苹果酸、2-氧代戊二酸、天冬氨酸、谷氨酸和柠檬酸的胞质和线粒体浓度的特征性变化上。数据表明，苹果酸-天冬氨酸穿梭是由胞质草酰乙酸浓度降低触发的，由于胞质天冬氨酸转氨酶平衡，这分别导致2-氧代戊二酸和天冬氨酸从线粒体中流出增加，以分别交换苹果酸和谷氨酸。柠檬酸循环被抑制的第一个位点似乎是2-氧代戊二酸氧化水平。
