Pavel S, Muskiet F A, Nagel G T, Schwippelová Z, Duchon J
J Chromatogr. 1981 Mar 13;222(3):329-36. doi: 10.1016/s0378-4347(00)84132-8.
The isolation of two Thormählen-positive compounds from the urine of a patient with malignant melanoma and the elucidation of their structure by gas chromatography-mass spectometry is described. The compounds were isolated using a poly-N-vinylpyrrolidone column and separated by preparative thin-layer chromatography. After elution they were analyzed by gas chromatography and gas chromatography-mass spectrometry as their trimethylsilyl derivatives and after hydrolysis also as their tert.-butyldimethylsilyl derivatives. The results showed the main Thormählen-positive compound A to be the glucuronide of 5-hydroxy-6-methoxyindole, whereas the minor compound AX appeared to be the glucuronide of its isomer 6-hydroxy-5-methoxyindole.
本文描述了从一名恶性黑色素瘤患者的尿液中分离出两种索姆尔伦阳性化合物，并通过气相色谱 - 质谱法阐明其结构的过程。这些化合物通过聚N - 乙烯基吡咯烷酮柱进行分离，并通过制备型薄层色谱法进一步分离。洗脱后，将它们作为三甲基硅烷基衍生物通过气相色谱和气相色谱 - 质谱法进行分析，水解后还作为叔丁基二甲基硅烷基衍生物进行分析。结果表明，主要的索姆尔伦阳性化合物A是5 - 羟基 - 6 - 甲氧基吲哚的葡萄糖醛酸苷，而次要化合物AX似乎是其异构体6 - 羟基 - 5 - 甲氧基吲哚的葡萄糖醛酸苷。
