Pavel S, Elzinga H, Muskiet F A, Smit J M, Mulder N H, Koops H S
J Clin Chem Clin Biochem. 1986 Mar;24(3):167-73. doi: 10.1515/cclm.1986.24.3.167.
A method was developed for the quantitation of six eumelanin-related indolic compounds in urine. The procedure employs methane ionization of the hexafluoroisopropyl and/or pentafluoropropionyl derivatives of 5,6-dihydroxyindole, 5-hydroxy-6-methoxyindole, 6-hydroxy-5-methoxyindole, 5-hydroxy-6-methoxyindolyl-2-carboxylic acid, 6-hydroxy-5-methoxyindolyl-2-carboxylic acid and 5,6-dimethoxyindolyl-2-carboxylic acid together with their deuterated analogues, and selected ion-monitoring. Deuterated analogues are added as internal standards to enzymatically hydrolysed urine before diethyl ether extraction. The method was used for monitoring urinary excretion in patients with malignant melanoma treated with cytostatics. In several cases, a decrease of the excretion of the eumelanin-related substances was recorded after the therapy. The usefulness of the individual indolic compounds for such follow-up studies is discussed.
已开发出一种用于定量尿液中六种与真黑素相关的吲哚化合物的方法。该程序采用对5,6 - 二羟基吲哚、5 - 羟基 - 6 - 甲氧基吲哚、6 - 羟基 - 5 - 甲氧基吲哚、5 - 羟基 - 6 - 甲氧基吲哚 - 2 - 羧酸、6 - 羟基 - 5 - 甲氧基吲哚 - 2 - 羧酸和5,6 - 二甲氧基吲哚 - 2 - 羧酸及其氘代类似物的六氟异丙基和/或五氟丙酰基衍生物进行甲烷离子化，并进行选择离子监测。在乙醚萃取前，将氘代类似物作为内标添加到酶解后的尿液中。该方法用于监测接受细胞抑制剂治疗的恶性黑色素瘤患者的尿排泄情况。在几例病例中，治疗后记录到与真黑素相关物质的排泄量减少。讨论了各个吲哚化合物在这类随访研究中的有用性。
