Pavel S, Muskiet F A, Budesínská A, Duchon J
Tumori. 1981 Aug;67(4):325-32. doi: 10.1177/030089168106700409.
A qualitative gas chromatographic analysis of trimethylsilylated ethyl acetate extracts of melanotic urine revealed 5 indolic compounds, which have been identified as substituted 5,6-dihydroxyindoles. Ethyl acetate extracts of melanotic urines at pH 2.0 contained isomeric 5-hydroxy-6-methoxy and 6-hydroxy-5-methoxy-indolyl-2-carboxylic acids which were not separable under the conditions used. A careful hydrolysis of melanotic urine with a Helix pomatia preparation followed by extraction at pH 6.5 in a nitrogen atmosphere released 3 additional indolic compounds from their conjugated form. Using gas chromatographic-mass spectrometric analysis they were identified as 5-hydroxy-6-methoxy, 6-hydroxy-5-methoxyindole and 5,6-dihydroxyindole.
对黑素尿的三甲基硅烷基化乙酸乙酯提取物进行的定性气相色谱分析显示有5种吲哚类化合物，已鉴定为取代的5,6 - 二羟基吲哚。pH 2.0的黑素尿的乙酸乙酯提取物含有异构的5 - 羟基 - 6 - 甲氧基和6 - 羟基 - 5 - 甲氧基 - 吲哚 - 2 - 羧酸，在所使用的条件下无法分离。用玛瑙螺制剂对黑素尿进行仔细水解，然后在氮气气氛中于pH 6.5下萃取，从其共轭形式中释放出另外3种吲哚类化合物。通过气相色谱 - 质谱分析，它们被鉴定为5 - 羟基 - 6 - 甲氧基、6 - 羟基 - 5 - 甲氧基吲哚和5,6 - 二羟基吲哚。
