Bailey E, Barron E J
J Chromatogr. 1980 Jul 11;183(1):25-31. doi: 10.1016/s0378-4347(00)81394-8.
A precise and sensitive method is described for the determination of the antidepressant drug tranylcypromine in human plasma and urine using high-resolution gas chromatography. The drug, together with an added internal standard, is extracted from the plasma or urine sample, derivatized with heptafluorobutyric anhydride and analysed on an OV-225 support-coated open-tubular glass capillary column with nitrogen-sensitive detection. The method has been applied to the measurement of tranylcypromine levels in plasma and urine from both healthy volunteers and from psychiatric patients receiving a therapeutic dosage of the drug.
描述了一种使用高分辨率气相色谱法测定人血浆和尿液中抗抑郁药物反苯环丙胺的精确且灵敏的方法。该药物与添加的内标物一起从血浆或尿液样品中提取出来，用七氟丁酸酐进行衍生化，然后在涂有OV - 225固定相的开口管式玻璃毛细管柱上进行分析，并采用氮敏感检测。该方法已应用于测定健康志愿者以及接受该药物治疗剂量的精神科患者血浆和尿液中的反苯环丙胺水平。
