de Savigny D, Voller A
J Immunoassay. 1980;1(1):105-28. doi: 10.1080/01971528008055779.
The data generated by quantitative enzyme-immunoassay of antibody activity are unsuitable for direct communication from the laboratory to clinician. In this paper, the fundamental problems of reporting ELISA results are highlighted and a list of desirable characteristics is set out for such reports. The available methods for processing ELISA data are reviewed in relation to these ideal requirements and the deficiencies and advantages of each method are discussed. The performance of each method is demonstrated by the use of actual test data from a model ELISA system. No single method has been found which satisfies all criteria.
通过定量酶免疫测定抗体活性所产生的数据不适合直接从实验室传达给临床医生。本文强调了报告酶联免疫吸附测定(ELISA)结果的基本问题,并列出了此类报告应具备的一系列理想特征。针对这些理想要求,对现有的ELISA数据处理方法进行了综述,并讨论了每种方法的优缺点。通过使用来自一个模型ELISA系统的实际测试数据展示了每种方法的性能。尚未发现一种能满足所有标准的单一方法。