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用于检测牛抗流产布鲁氏菌抗体的特异性酶联免疫吸附测定

Specific enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus.

作者信息

Tabatabai L B, Deyoe B L

出版信息

J Clin Microbiol. 1984 Aug;20(2):209-13. doi: 10.1128/jcm.20.2.209-213.1984.

DOI:10.1128/jcm.20.2.209-213.1984
PMID:6436294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271288/
Abstract

Six soluble antigens prepared from Brucella abortus were compared with a salt-extractable protein (CSP) antigen in an enzyme-linked immunosorbent assay for the detection of antibody to B. abortus in cattle sera. Of seven preparations tested, antigens from B. abortus soluble antigen (prepared from an autoclaved cell suspension) and CSP were stable on frozen storage. Enzyme-linked immunosorbent assay with CSP antigen under optimal conditions was from 100- to 700-fold more sensitive than the standard agglutination, card, Rivanol precipitation-plate agglutination, and the complement fixation tests in detecting immunoglobulin G antibody. From a practical point of view, however, using the most stringent criteria for determining an "upper negative" value, the enzyme-linked immunosorbent assay with CSP was at least 12-fold more sensitive than the standard agglutination test and any of the other serological tests. Furthermore, the enzyme-linked immunosorbent assay with CSP was specific for antibody to B. abortus.

摘要

在一项酶联免疫吸附试验中，对从流产布鲁氏菌制备的六种可溶性抗原与一种盐可提取蛋白（CSP）抗原进行了比较，以检测牛血清中抗流产布鲁氏菌的抗体。在测试的七种制剂中，流产布鲁氏菌可溶性抗原（由高压灭菌的细胞悬液制备）和CSP抗原在冷冻保存时稳定。在最佳条件下，用CSP抗原进行的酶联免疫吸附试验在检测免疫球蛋白G抗体方面比标准凝集试验、卡片试验、利凡诺沉淀平板凝集试验和补体结合试验敏感100至700倍。然而，从实际角度来看，使用最严格的标准来确定“上限阴性”值时，用CSP进行的酶联免疫吸附试验比标准凝集试验和任何其他血清学试验至少敏感12倍。此外，用CSP进行的酶联免疫吸附试验对流产布鲁氏菌抗体具有特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1e/271288/3ad52ee5462b/jcm00121-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1e/271288/3ad52ee5462b/jcm00121-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1e/271288/3ad52ee5462b/jcm00121-0091-a.jpg

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