破伤风抗体快速定量微酶联免疫吸附测定法
Rapid quantitative microenzyme-linked immunosorbent assay for tetanus antibodies.
作者信息
Sedgwick A K, Ballow M, Sparks K, Tilton R C
出版信息
J Clin Microbiol. 1983 Jul;18(1):104-9. doi: 10.1128/jcm.18.1.104-109.1983.
Abstract
A rapid quantitative microenzyme-linked immunosorbent assay for the detection of immunoglobulin G antibodies to tetanus toxoid is described. The assay is performed in 2.5 h and detects antibody levels of between 0.1 and 6 U/ml. Quantitation of the antibody concentration is unique in that multiple serum dilutions are tested on a single patient, the results are plotted as an antibody dilution curve, and the area under the curve is integrated for a final concentration. Tests for immune status on sera from 77 individuals demonstrated tetanus antibody concentrations of greater than or equal to 0.1 U/ml in 92% of a pediatric group and 62% of an adult group. The microenzyme-linked immunosorbent assay procedure is reproducible and is useful for the determination of immune status and immunization response.
摘要
本文描述了一种用于检测破伤风类毒素免疫球蛋白G抗体的快速定量微酶联免疫吸附测定法。该测定法在2.5小时内完成，可检测0.1至6 U/ml之间的抗体水平。抗体浓度的定量方法独特，即对单个患者的多种血清稀释液进行检测，将结果绘制成抗体稀释曲线，并对曲线下面积进行积分以得出最终浓度。对77名个体的血清进行免疫状态检测，结果显示，儿科组92%和成人组62%的破伤风抗体浓度大于或等于0.1 U/ml。微酶联免疫吸附测定法具有可重复性，可用于确定免疫状态和免疫反应。
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Immunoassay using antigen-enzyme conjugates.使用抗原-酶偶联物的免疫测定法。
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A rapid automated latex screen for tetanus toxoid antibodies.一种用于破伤风类毒素抗体的快速自动化乳胶筛查。
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