Cohen S R, Burkholder D E, Varga J M, Carter D M, Bartholomew J C
J Invest Dermatol. 1981 May;76(5):409-13. doi: 10.1111/1523-1747.ep12520956.
Cell cycle analysis was used to study the the effect of 4,5'8-trimethylpsoralen (TMP) and long-wave ultraviolet light (UV-A) on cultured mammalian cells. DNA distribution patterns were measured for murine melanoma cells (a cloned line of Cloudman S91) and a strain of diploid human skin fibroblasts (CRL 1295) using both a microfluorimetry procedure and flow cytometry. The untreated cells and those receiving TMP along and UV-A alone had identical DNA content as assessed at several posttreatment intervals (0-72 hr). The majority of cells in control groups contained a G1 DNA content, whereas exposure to TMP (2 x 10(-7) M) plus UV-A (1 Joule/cm2) led to the accumulation of cells in the G2 phase. These observations were similar for each cell type and both analytical techniques were in excellent agreement. The finding that psoralen plus UV-A induces a phase-specific G2 blockade in cultured cells has important implications for understanding the mechanisms which account for enhanced pigmentation and suppression of cellular proliferation following exposure to these agents in vivo.
采用细胞周期分析方法研究了4,5',8-三甲基补骨脂素（TMP）和长波紫外线（UV-A）对培养的哺乳动物细胞的影响。使用微量荧光测定法和流式细胞术测量了小鼠黑色素瘤细胞（Cloudman S91克隆系）和一株二倍体人皮肤成纤维细胞（CRL 1295）的DNA分布模式。在几个处理后时间间隔（0 - 72小时）评估时，未处理的细胞以及仅接受TMP或仅接受UV-A的细胞具有相同的DNA含量。对照组中的大多数细胞含有G1期DNA含量，而暴露于TMP（2×10⁻⁷ M）加UV-A（1焦耳/平方厘米）会导致细胞在G2期积累。每种细胞类型的这些观察结果相似，并且两种分析技术结果高度一致。补骨脂素加UV-A在培养细胞中诱导阶段特异性G2期阻滞这一发现，对于理解体内暴露于这些试剂后色素沉着增强和细胞增殖受抑制的机制具有重要意义。
