Raine T J, Heggers J P, Robson M C, London M D, Johns L
J Trauma. 1981 May;21(5):394-7. doi: 10.1097/00005373-198105000-00012.
To evaluate the effect which cooling of burn wounds has upon dermal microvascular circulation, standard 10% BSA dorsal guinea-pig scald burns were created. In treated animals, the dorsal burned region was immersed in a circulating ice-water bath (0-3 degrees C) for 30 minutes at various time intervals postburn (10,20,30, and 60 minutes after scald). India-ink perfusion was used to determine the level of dermal vessel patency at 2, 4, 8, 24, 72, and 96 hours after burning. By 96 hours, significant differences could be seen in animals cooled 10, 20, or 30 minutes after burning. Those not treated until 60 minutes after burning had dermal perfusion essentially the same as control animals. 133Xe dermal washout studies were performed at 8 and 24 hours and corroborated these findings. Grossly, burn wounds cooled by 30 minutes after burn had markedly better healing than control animals and animals cooled 60 minutes after burning. We believe these data support the early use of cold water treatment of partial-thickness burn wounds, as demonstrated in this experimental study.
为评估烧伤创面冷却对真皮微血管循环的影响，制作了标准的10%体表面积的豚鼠背部烫伤模型。对于接受治疗的动物，在烧伤后的不同时间间隔（烫伤后10、20、30和60分钟），将背部烧伤区域浸入循环冰水浴（0 - 3摄氏度）中30分钟。采用印度墨汁灌注法测定烧伤后2、4、8、24、72和96小时真皮血管通畅程度。到96小时时，在烧伤后10、20或30分钟进行冷却处理的动物中可观察到显著差异。那些直到烧伤后60分钟才接受治疗的动物，其真皮灌注情况与对照动物基本相同。在8小时和24小时进行了¹³³Xe真皮洗脱研究，证实了这些结果。大体观察，烧伤后30分钟进行冷却处理的烧伤创面愈合情况明显优于对照动物以及烧伤后60分钟进行冷却处理的动物。我们认为，如本实验研究所证明的，这些数据支持早期使用冷水处理浅度烧伤创面。
