一种用于测量遥甲基组胺的改进型气相色谱-质谱联用（GCMS）方法。

An improved GCMS method to measure tele-methylhistamine.

作者信息

Hough L B, Khandelwal J K, Morrishow A M, Green J P

出版信息

J Pharmacol Methods. 1981 Mar;5(2):143-8. doi: 10.1016/0160-5402(81)90006-1.

Abstract

The presence of tele-methylhistamine (t-MH), the metabolite of histamine in rat brain, and its relationship to putative histaminergic transmission have been the subjects of recent work. We modified the GCMS method of Hough et al. (1979) to enhance both sensitivity and reproducibility. The substitution of KOH for NaOH to extract t-MH considerably improved the recovery. Evaporation of the extract in 0.2 N HCl, as used in the earlier method, reduced the formation of heptafluorobutyryl derivative; substitution with 0.01 N HCl more than doubled the recovery of the derivative. The derivatization procedure itself was changed, the new method exhibiting significantly improved reproducibility. Standard curve of t-MH obtained at different times after derivatization were indistinguishable. The modified method is capable of measuring less than 1 pmole of t-MH. The t-MH content found in nine rat brain regions agree with previously reported values.

摘要

大鼠脑中组胺的代谢产物——远甲基组胺（t-MH）的存在及其与假定的组胺能传递的关系一直是近期研究的主题。我们改进了霍夫等人（1979年）的气相色谱 - 质谱联用（GCMS）方法，以提高灵敏度和重现性。用氢氧化钾替代氢氧化钠来提取t-MH显著提高了回收率。如早期方法那样在0.2N盐酸中蒸发提取物会减少七氟丁酰衍生物的形成；用0.01N盐酸替代使衍生物的回收率提高了一倍多。衍生化程序本身也有所改变，新方法的重现性显著提高。衍生化后不同时间获得的t-MH标准曲线没有差异。改进后的方法能够检测到少于1皮摩尔的t-MH。在九个大鼠脑区中发现的t-MH含量与先前报道的值相符。