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脊椎动物组蛋白基因转录发生在两条DNA链上。

Vertebrate histone gene transcription occurs from both DNA strands.

作者信息

Bruschi S, Wells J R

出版信息

Nucleic Acids Res. 1981 Apr 10;9(7):1591-7. doi: 10.1093/nar/9.7.1591.

DOI:10.1093/nar/9.7.1591
PMID:7232222
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326784/
Abstract

Three clones containing vertebrate histone genes, two from chicken and one from human have been investigated. DNA from each recombinant within lambda Charon 4A has been strand-separated on agarose gels in the presence of poly r(UG). Southern transfer analysis with histone cDNA indicates that in each case transcription occurs from both strands. These results argue against the possibility of polycistronic transcription of these vertebrate histone genes.

摘要

已经对三个含有脊椎动物组蛋白基因的克隆进行了研究,其中两个来自鸡,一个来自人。λ噬菌体Charon 4A中每个重组体的DNA在聚r(UG)存在下于琼脂糖凝胶上进行了链分离。用组蛋白cDNA进行的Southern转移分析表明,在每种情况下两条链都发生转录。这些结果反对了这些脊椎动物组蛋白基因多顺反子转录的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb2c/326784/025d67a9a0f1/nar00400-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb2c/326784/9540dc01a196/nar00400-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb2c/326784/025d67a9a0f1/nar00400-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb2c/326784/9540dc01a196/nar00400-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb2c/326784/025d67a9a0f1/nar00400-0066-a.jpg

相似文献

1
Vertebrate histone gene transcription occurs from both DNA strands.脊椎动物组蛋白基因转录发生在两条DNA链上。
Nucleic Acids Res. 1981 Apr 10;9(7):1591-7. doi: 10.1093/nar/9.7.1591.
2
Isolation of a clone containing human histone genes.包含人类组蛋白基因的克隆的分离。
Nucleic Acids Res. 1981 Apr 10;9(7):1583-90. doi: 10.1093/nar/9.7.1583.
3
Histone genes are clustered but not tandemly repeated in the chicken genome.组蛋白基因在鸡基因组中是成簇排列的,但不是串联重复的。
Proc Natl Acad Sci U S A. 1981 May;78(5):2856-60. doi: 10.1073/pnas.78.5.2856.
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Genomic organization, DNA sequence, and expression of chicken embryonic histone genes.鸡胚胎组蛋白基因的基因组组织、DNA序列及表达
J Biol Chem. 1983 Jul 25;258(14):9005-16.
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The histone H5 gene is flanked by S1 hypersensitive structures.组蛋白H5基因两侧是S1超敏结构。
Nucleic Acids Res. 1984 Aug 24;12(16):6473-92. doi: 10.1093/nar/12.16.6473.
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The chicken H5 gene is unlinked to core and H1 histone genes.鸡的H5基因与核心组蛋白基因和H1组蛋白基因不连锁。
Nucleic Acids Res. 1983 Feb 11;11(3):619-27. doi: 10.1093/nar/11.3.619.
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Isolation of a genomal clone containing chicken histone genes.包含鸡组蛋白基因的基因组克隆的分离
Nucleic Acids Res. 1979 Dec 11;7(7):1787-98. doi: 10.1093/nar/7.7.1787.
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Complete nucleotide sequence of a chicken H2b histone gene.鸡H2b组蛋白基因的完整核苷酸序列
J Biol Chem. 1982 Aug 10;257(15):8577-80.
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Chicken histone H5: selection of a cDNA recombinant using an extended synthetic primer.鸡组蛋白H5:使用延伸合成引物筛选cDNA重组体。
Nucleic Acids Res. 1982 Mar 11;10(5):1495-502. doi: 10.1093/nar/10.5.1495.
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The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences.粗糙脉孢菌中编码组蛋白H3和H4的基因是独特的,并且含有间隔序列。
Nucleic Acids Res. 1983 Aug 25;11(16):5347-60. doi: 10.1093/nar/11.16.5347.

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本文引用的文献

1
Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.海胆组蛋白mRNA末端位于假定调控序列下游的基因区域。
Nature. 1980 May 15;285(5761):147-51. doi: 10.1038/285147a0.
2
Chicken globin genes. Nucleotide sequence of cDNA clones coding for the alpha-globin expressed during hemolytic anemia.鸡珠蛋白基因。编码溶血性贫血期间表达的α-珠蛋白的cDNA克隆的核苷酸序列。
J Biol Chem. 1980 Oct 10;255(19):9306-11.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
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Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.海胆组蛋白基因簇的分子分析:III. 组蛋白编码序列的极性与不对称性
Cell. 1976 Aug;8(4):479-84. doi: 10.1016/0092-8674(76)90215-4.
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Presence of histone mRNA sequences in high molecular weight RNA of HeLa cells.
Cell. 1977 Jul;11(3):651-61. doi: 10.1016/0092-8674(77)90082-4.
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The histone genes in HeLa cells are on individual transcriptional units.
J Mol Biol. 1978 Dec 25;126(4):619-35. doi: 10.1016/0022-2836(78)90012-8.
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Short half-life precursor of globin messenger RNA from chicken erythroblasts.来自鸡成红细胞的珠蛋白信使核糖核酸的短半衰期前体。
Biochemistry. 1978 May 2;17(9):1591-6. doi: 10.1021/bi00602a002.
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Isolation of yeast histone genes H2A and H2B.酵母组蛋白基因H2A和H2B的分离
Cell. 1979 Dec;18(4):1261-71. doi: 10.1016/0092-8674(79)90237-x.
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Histone genes are clustered with a 15-kilobase repeat in the chicken genome.
Nature. 1979 May 10;279(5709):132-6. doi: 10.1038/279132a0.
10
Structure of chromatin.染色质的结构。
Annu Rev Biochem. 1977;46:931-54. doi: 10.1146/annurev.bi.46.070177.004435.