脊椎动物组蛋白基因转录发生在两条DNA链上。

Vertebrate histone gene transcription occurs from both DNA strands.

作者信息

Bruschi S, Wells J R

出版信息

Nucleic Acids Res. 1981 Apr 10;9(7):1591-7. doi: 10.1093/nar/9.7.1591.

DOI:10.1093/nar/9.7.1591

PMID:7232222

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326784/

Abstract

Three clones containing vertebrate histone genes, two from chicken and one from human have been investigated. DNA from each recombinant within lambda Charon 4A has been strand-separated on agarose gels in the presence of poly r(UG). Southern transfer analysis with histone cDNA indicates that in each case transcription occurs from both strands. These results argue against the possibility of polycistronic transcription of these vertebrate histone genes.

摘要

已经对三个含有脊椎动物组蛋白基因的克隆进行了研究，其中两个来自鸡，一个来自人。λ噬菌体Charon 4A中每个重组体的DNA在聚r（UG）存在下于琼脂糖凝胶上进行了链分离。用组蛋白cDNA进行的Southern转移分析表明，在每种情况下两条链都发生转录。这些结果反对了这些脊椎动物组蛋白基因多顺反子转录的可能性。