• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

包含鸡组蛋白基因的基因组克隆的分离

Isolation of a genomal clone containing chicken histone genes.

作者信息

Harvey R P, Wells J R

出版信息

Nucleic Acids Res. 1979 Dec 11;7(7):1787-98. doi: 10.1093/nar/7.7.1787.

DOI:10.1093/nar/7.7.1787
PMID:161399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342348/
Abstract

We have used enriched chicken histone cDNA to select genomal clones from a chicken library. Because the cDNA probe also contained other sequences, a further screening of positive plagues with negative probes eliminated most non-histone gene clones. One 'positively-selected' genomal clone, lambda CH-01, hybridised with cloned sea-urchin histone genes and also detected histone genes in EcoRI-digested genomal sea-urchin DNA. Limited DNA sequencing of HaeIII fragments identified two sequences within the coding region of chicken histone H2A. A third fragment predicted an amino acid sequence with strong homology to an H1 histone sequence.

摘要

我们利用富集的鸡组蛋白cDNA从鸡文库中筛选基因组克隆。由于cDNA探针还包含其他序列，用阴性探针进一步筛选阳性噬菌斑可去除大多数非组蛋白基因克隆。一个“正向选择”的基因组克隆λCH-01，与克隆的海胆组蛋白基因杂交，并且在经EcoRI酶切的海胆基因组DNA中也检测到了组蛋白基因。对HaeIII片段进行有限的DNA测序，确定了鸡组蛋白H2A编码区内的两个序列。第三个片段预测的氨基酸序列与H1组蛋白序列有很强的同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/6fa9713e556b/nar00460-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/ca1666254fe7/nar00460-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/9663f8ecac7e/nar00460-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/0d711086ec3e/nar00460-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/6fa9713e556b/nar00460-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/ca1666254fe7/nar00460-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/9663f8ecac7e/nar00460-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/0d711086ec3e/nar00460-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d5/342348/6fa9713e556b/nar00460-0064-a.jpg

相似文献

1
Isolation of a genomal clone containing chicken histone genes.包含鸡组蛋白基因的基因组克隆的分离
Nucleic Acids Res. 1979 Dec 11;7(7):1787-98. doi: 10.1093/nar/7.7.1787.
2
Isolation of a clone containing human histone genes.包含人类组蛋白基因的克隆的分离。
Nucleic Acids Res. 1981 Apr 10;9(7):1583-90. doi: 10.1093/nar/9.7.1583.
3
Histone genes are clustered but not tandemly repeated in the chicken genome.组蛋白基因在鸡基因组中是成簇排列的，但不是串联重复的。
Proc Natl Acad Sci U S A. 1981 May;78(5):2856-60. doi: 10.1073/pnas.78.5.2856.
4
Genomic organization, DNA sequence, and expression of chicken embryonic histone genes.鸡胚胎组蛋白基因的基因组组织、DNA序列及表达
J Biol Chem. 1983 Jul 25;258(14):9005-16.
5
Vertebrate histone genes: nucleotide sequence of a chicken H2A gene and regulatory flanking sequences.脊椎动物组蛋白基因：鸡H2A基因的核苷酸序列及侧翼调控序列
Nucleic Acids Res. 1981 Jul 10;9(13):3119-28. doi: 10.1093/nar/9.13.3119.
6
H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.H2A.F：一种在鸡胚胎中表达的极其变异的组蛋白H2A序列。
Proc Natl Acad Sci U S A. 1983 May;80(10):2819-23. doi: 10.1073/pnas.80.10.2819.
7
The chicken H5 gene is unlinked to core and H1 histone genes.鸡的H5基因与核心组蛋白基因和H1组蛋白基因不连锁。
Nucleic Acids Res. 1983 Feb 11;11(3):619-27. doi: 10.1093/nar/11.3.619.
8
Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5' region of H5 mRNA.构建含有来自母鸡红细胞组蛋白H5 cDNA的嵌合质粒。源自H5 mRNA 5'区域的片段的DNA序列。
Nucleic Acids Res. 1982 Mar 25;10(6):2093-108. doi: 10.1093/nar/10.6.2093.
9
Chicken histone H5: selection of a cDNA recombinant using an extended synthetic primer.鸡组蛋白H5：使用延伸合成引物筛选cDNA重组体。
Nucleic Acids Res. 1982 Mar 11;10(5):1495-502. doi: 10.1093/nar/10.5.1495.
10
Organization of human histone genes.人类组蛋白基因的组织
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1795-9. doi: 10.1073/pnas.79.6.1795.

引用本文的文献

1
Vertebrate histone gene transcription occurs from both DNA strands.脊椎动物组蛋白基因转录发生在两条DNA链上。
Nucleic Acids Res. 1981 Apr 10;9(7):1591-7. doi: 10.1093/nar/9.7.1591.
2
Structure and in vitro transcription of a human H4 histone gene.人类H4组蛋白基因的结构与体外转录
Nucleic Acids Res. 1983 Oct 25;11(20):7069-86. doi: 10.1093/nar/11.20.7069.
3
The structural organization and DNA sequence of a wheat histone H4 gene.一个小麦组蛋白H4基因的结构组织与DNA序列

本文引用的文献

1
A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6. doi: 10.1016/0006-291x(66)90447-5.
2
Qualitative species differences and quantitative tissue differences in the distribution of lysine-rich histones.富含赖氨酸组蛋白分布中的定性物种差异和定量组织差异。
J Biol Chem. 1969 Jun 25;244(12):3375-86.
3
Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger.具有组蛋白信使特性的快速标记的多核糖体相关RNA。
Nucleic Acids Res. 1983 Sep 10;11(17):5865-75. doi: 10.1093/nar/11.17.5865.
4
Organization of human histone genes.人类组蛋白基因的组织
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1795-9. doi: 10.1073/pnas.79.6.1795.
5
Vertebrate histone genes: nucleotide sequence of a chicken H2A gene and regulatory flanking sequences.脊椎动物组蛋白基因：鸡H2A基因的核苷酸序列及侧翼调控序列
Nucleic Acids Res. 1981 Jul 10;9(13):3119-28. doi: 10.1093/nar/9.13.3119.
6
Isolation of a clone containing human histone genes.包含人类组蛋白基因的克隆的分离。
Nucleic Acids Res. 1981 Apr 10;9(7):1583-90. doi: 10.1093/nar/9.7.1583.
7
Organization of the histone genes in the rainbow trout (Salmo gairdnerii).虹鳟鱼（Salmo gairdnerii）中组蛋白基因的组织方式。
J Mol Evol. 1984;20(3-4):227-35. doi: 10.1007/BF02104729.
8
Genetic markers on chromosome 7.7号染色体上的遗传标记。
J Med Genet. 1988 May;25(5):294-306. doi: 10.1136/jmg.25.5.294.
Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977-83. doi: 10.1073/pnas.58.5.1977.
4
Biogenesis and characterization of histone messenger RNA in HeLa cells.HeLa细胞中组蛋白信使核糖核酸的生物合成与特性分析
J Mol Biol. 1972 Jun 28;67(3):397-406. doi: 10.1016/0022-2836(72)90458-5.
5
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
6
Chromosomal proteins and chromatin structure.染色体蛋白与染色质结构。
Annu Rev Biochem. 1975;44:725-74. doi: 10.1146/annurev.bi.44.070175.003453.
7
Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliaris.海胆沙钱克隆组蛋白DNA的部分变性图谱分析
Nature. 1976 Nov 4;264(5581):31-4. doi: 10.1038/264031a0.
8
Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.海胆组蛋白基因簇的分子分析：III. 组蛋白编码序列的极性与不对称性
Cell. 1976 Aug;8(4):479-84. doi: 10.1016/0092-8674(76)90215-4.
9
Histone messengers and histone genes.组蛋白信使核糖核酸与组蛋白基因
Cell. 1976 Jul;8(3):321-31. doi: 10.1016/0092-8674(76)90144-6.
10
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule.富含赖氨酸的组蛋白H1在真核生物染色质中的作用及作用模式研究。组蛋白H1分子的三个结构区域。
Eur J Biochem. 1977 Jul 1;77(1):45-51. doi: 10.1111/j.1432-1033.1977.tb11639.x.