Dishon T, Slutzky G M, El-On J, Greenblatt C L
Isr J Med Sci. 1981 Apr;17(4):245-8.
The methods of coagglutination and indirect hemagglutination were used to detect the production of the immunologically active excreted factor (EF) of Leishmania. Staphylococci, rich in protein A and sensitized with specific anti-Leishmania antibodies, coagglutinated with supernatant fractions of cultures, thus enabling continuous monitoring of the excretion of EF by multiplying parasites. Papain-treated human red blood cells, sensitized with crude or purified EF, also agglutinated with the coagglutination reagent. The sensitized papain-treated red blood cells may be employed in indirect hemagglutination to detect specific antibodies to Leishmania in rabbit and human sera. As the EF is specific for each Leishmania serotype group, coagglutination and indirect hemagglutination offer the possibility of rapid, easy, sensitive and specific diagnostic tools in the determinations of both antigen and antibody in specimens from suspected cases of leishmaniasis.
采用协同凝集和间接血凝方法检测利什曼原虫免疫活性分泌因子（EF）的产生。富含A蛋白并经特异性抗利什曼原虫抗体致敏的葡萄球菌，可与培养物的上清组分发生协同凝集反应，从而能够对增殖的寄生虫分泌EF的情况进行持续监测。经木瓜蛋白酶处理的人红细胞，用粗制或纯化的EF致敏后，也能与协同凝集试剂发生凝集反应。致敏的经木瓜蛋白酶处理的红细胞可用于间接血凝，以检测兔和人血清中针对利什曼原虫的特异性抗体。由于EF对每种利什曼原虫血清型组具有特异性，协同凝集和间接血凝为利什曼病疑似病例标本中抗原和抗体的检测提供了快速、简便、灵敏且特异的诊断工具。
