Zeiss C R, Pruzansky J J, Levitz D, Wang J
Immunology. 1978 Aug;35(2):237-46.
IgE antibody specific for AgE (IgE—AgE) was eluted from human basophils at acid pH and quantified by its binding of I AgE in antigen excess. The quantity of Ige—AgE recovered from 30 ml of blood ranged from 0.08 ng to 10.3 ng representing 500 to 56,000 molecules IgE—AgE per basophil. The number of molecules of IgE—AgE per basophil was compared to plasma IgE—AgE, total plasma IgE and leucocyte histamine release in response to AgE. The ratio of plasma IgE—AgE to basophil bound IgE—AgE ranged from 100 to 4000, indicating that there are a limited number of IgE receptors on the basophil surface as contrasted to the concentration of IgE in the plasma. There was no correlation between IgE—AgE in plasma and the number of molecules of IgE—AgE per basophil. However there was a significant correlation between the ration of IgE—AgE to total IgE in plasma and the number of IgE—AgE molecules per basophil. Two measures of leucocyte histamine release in response to AgE, cell reactivity (maximum per cent histamine release attainable) and sensitivity (lowest antigen dose leading to 50% release), were compared to the number of IgE—AgE molecules per basophil. Cell reactivity was dependent on the number of IgE—AgE molecules per basophil. Only 2500 molecules IgE—AgE per basophil were required to reach a cellular reactivity of 50%. Cell sensitivity to AgE was not correlated with the number of molecules IgE—AgE per basophil which indicated that other factors played a role in determining the sensitivity of a population of basophils to antigenic stimulation by AgE.
针对变应原E（IgE - AgE）的IgE抗体在酸性pH条件下从人嗜碱性粒细胞中洗脱出来，并通过其在抗原过量时与1个变应原E的结合来定量。从30毫升血液中回收的IgE - AgE量在0.08纳克至10.3纳克之间，相当于每个嗜碱性粒细胞有500至56,000个IgE - AgE分子。将每个嗜碱性粒细胞的IgE - AgE分子数与血浆IgE - AgE、血浆总IgE以及对变应原E反应时白细胞组胺释放量进行比较。血浆IgE - AgE与嗜碱性粒细胞结合的IgE - AgE的比例在100至4000之间，这表明与血浆中IgE的浓度相比，嗜碱性粒细胞表面的IgE受体数量有限。血浆中的IgE - AgE与每个嗜碱性粒细胞的IgE - AgE分子数之间没有相关性。然而，血浆中IgE - AgE与总IgE的比例与每个嗜碱性粒细胞的IgE - AgE分子数之间存在显著相关性。将对变应原E反应时白细胞组胺释放的两项指标，即细胞反应性（可达到的最大组胺释放百分比）和敏感性（导致50%释放的最低抗原剂量）与每个嗜碱性粒细胞的IgE - AgE分子数进行比较。细胞反应性取决于每个嗜碱性粒细胞的IgE - AgE分子数。每个嗜碱性粒细胞仅需2500个IgE - AgE分子就能达到50%的细胞反应性。细胞对变应原E的敏感性与每个嗜碱性粒细胞的IgE - AgE分子数无关，这表明其他因素在决定嗜碱性粒细胞群体对抗变应原E抗原刺激的敏感性方面发挥了作用。
