Musoke A J, Nantulya V M, Barbet A F, Kironde F, McGuire T C
Parasite Immunol. 1981 Summer;3(2):97-106. doi: 10.1111/j.1365-3024.1981.tb00388.x.
Cattle were infected with two different clones of Trypanosoma brucei (MITat 1.2 and ILTat 1.3) and antibody response was followed by radioimmunoassay. In four of the seven animals there were at least two peaks of antibody activity to the infecting clones, with the second peak much higher than the first. Specific antibodies (IgG1 and IgM but not IgG2) were eluted from the immunoabsorbent columns on which the variant surface glycoproteins (VSGs) were coupled. By neutralization of infectivity tests, IgM antibodies from the first peak of antibody activity were more efficient in neutralizing trypanosomes than IgG1 but the reverse was true of the antibodies isolated from the second peak. By absorption with multiple variable antigen types isolated during the course of infection, all the IgM and IgG1 in the first 3 weeks of infection were shown to be specific. It is suggested that polyclonal B cell stimulation leading to dysfunction in the control of IgM and IgG production may not be responsible for the high levels of these immunoglobulins in bovine trypanosomiasis.
牛感染了两种不同克隆的布氏锥虫（MITat 1.2和ILTat 1.3），并通过放射免疫测定法跟踪抗体反应。在七只动物中的四只中，针对感染克隆的抗体活性至少有两个峰值，第二个峰值远高于第一个峰值。从偶联了变异表面糖蛋白（VSG）的免疫吸附柱上洗脱了特异性抗体（IgG1和IgM，但不是IgG2）。通过感染性中和试验，抗体活性第一个峰值的IgM抗体在中和锥虫方面比IgG1更有效，但从第二个峰值分离的抗体情况则相反。通过用感染过程中分离的多种可变抗原类型进行吸收，结果表明感染前三周内所有的IgM和IgG1都是特异性的。有人提出，导致IgM和IgG产生控制功能失调的多克隆B细胞刺激可能不是牛锥虫病中这些免疫球蛋白水平升高的原因。
