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补骨脂素 - DNA光反应：利用纳秒紫外激光脉冲控制单加合物和双加合物的产生。

Psoralen-DNA photoreaction: controlled production of mono- and diadducts with nanosecond ultraviolet laser pulses.

作者信息

Johnston B H, Johnson M A, Moore C B, Hearst J E

出版信息

Science. 1977 Aug 26;197(4306):906-8. doi: 10.1126/science.887929.

DOI:10.1126/science.887929
PMID:887929
Abstract

Samples of DNA which contain adducts of a new psoralen derivative, but no cross-links, have been prepared by irradiating mixtures of DNA and the derivative with single, 15-nanosecond pulses of laser light. Succeeding pulses introduce cross-links. The ability to rapidly and selectively create monoadducts and corss-links may allow the use of psoralens as probes of dynamic processes in living cells.

摘要

通过用单个15纳秒的激光脉冲照射DNA与该衍生物的混合物，制备出了含有一种新的补骨脂素衍生物加合物但没有交联的DNA样本。后续的脉冲会引入交联。快速且选择性地产生单加合物和交联的能力可能使补骨脂素能够用作活细胞中动态过程的探针。

相似文献

1
Psoralen-DNA photoreaction: controlled production of mono- and diadducts with nanosecond ultraviolet laser pulses.补骨脂素 - DNA光反应：利用纳秒紫外激光脉冲控制单加合物和双加合物的产生。
Science. 1977 Aug 26;197(4306):906-8. doi: 10.1126/science.887929.
2
Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.具有与DNA和RNA更高光反应活性的新型补骨脂素衍生物的合成与表征
Biochemistry. 1977 Mar 22;16(6):1058-64. doi: 10.1021/bi00625a005.
3
Photoreactivity of furocoumarins and DNA in PUVA therapy: formation of psoralen-thymine adducts.补骨脂素与DNA在光化学疗法中的光反应性：补骨脂素 - 胸腺嘧啶加合物的形成。
J Phys Chem B. 2008 Nov 6;112(44):14002-10. doi: 10.1021/jp805523d. Epub 2008 Oct 15.
4
Binding of psoralen derivatives to DNA and chromatin: influence of the ionic environment on dark binding and photoreactivity.补骨脂素衍生物与DNA和染色质的结合：离子环境对暗结合和光反应性的影响。
Biochemistry. 1978 Apr 4;17(7):1251-7. doi: 10.1021/bi00600a019.
5
Genetic effects of photoadducts and photocross-links in the DNA of phage lambda exposed to 360 nm light and tri-methylpsoralen or khellin.λ噬菌体DNA中光加合物和光交联在暴露于360纳米光及三甲基补骨脂素或凯林时的遗传效应
Biochim Biophys Acta. 1977 Apr 19;475(4):589-600. doi: 10.1016/0005-2787(77)90319-7.
6
Mutagenicity of cross-links and monoadducts of furocoumarins (psoralen and angelicin) induced by 360-nm radiation in excision-repair-defective and radiation-insensitive strains of Saccharomyces cerevisiae.360纳米辐射诱导的呋喃香豆素（补骨脂素和白芷素）交联物与单加合物在酿酒酵母切除修复缺陷及辐射不敏感菌株中的致突变性
Environ Mutagen. 1979;1(1):55-63. doi: 10.1002/em.2860010112.
7
Psoralen-crosslinked secondary structure map of single-stranded virus DNA.单链病毒DNA的补骨脂素交联二级结构图谱。
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2649-53. doi: 10.1073/pnas.73.8.2649.
8
Kinetics of formation of deoxyribonucleic acid cross-links by 4'-(aminomethyl)-4,5',8-trimethylpsoralen.4'-（氨甲基）-4,5',8-三甲基补骨脂素形成脱氧核糖核酸交联的动力学
Biochemistry. 1981 Feb 17;20(4):735-8. doi: 10.1021/bi00507a011.
9
Spectroscopic study of the dar interaction and of the photoreaction between a new monofunctional psoralen: 3-carbethoxy psoralen, and DNA.新型单官能团补骨脂素：3-乙氧羰基补骨脂素与DNA之间的相互作用及光反应的光谱研究
Biochimie. 1981 Nov-Dec;63(11-12):899-905. doi: 10.1016/s0300-9084(82)80283-6.
10
[Electrophoresis of DNA fragments renatured and cross-linked with psoralen: kinetic analysis of DNA photocross-linking].[用补骨脂素复性和交联的DNA片段的电泳：DNA光交联的动力学分析]
Mol Biol (Mosk). 1986 Jul-Aug;20(4):1039-47.

引用本文的文献

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Micro-irradiation tools to visualize base excision repair and single-strand break repair.用于可视化碱基切除修复和单链断裂修复的微辐射工具。
DNA Repair (Amst). 2015 Jul;31:52-63. doi: 10.1016/j.dnarep.2015.05.001. Epub 2015 May 5.
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Repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells.G1期哺乳动物细胞中激光定位的DNA链间交联的修复
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DNA structural reorganization upon conversion of a psoralen furan-side monoadduct to an interstrand cross-link: implications for DNA repair.补骨脂素呋喃侧单加合物转化为链间交联时的DNA结构重组：对DNA修复的影响
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2345-9. doi: 10.1073/pnas.92.6.2345.
5
Double stranded scission of DNA directed through sequence-specific R-loop formation.通过序列特异性R环形成介导的DNA双链断裂。
Nucleic Acids Res. 1995 Sep 11;23(17):3524-30. doi: 10.1093/nar/23.17.3524.
6
Viroids and prions.类病毒和朊病毒。
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5220-4. doi: 10.1073/pnas.79.17.5220.
7
Termination sites of the in vitro nick-translation reaction on DNA that had photoreacted with psoralen.与补骨脂素发生光反应的DNA上体外切口平移反应的终止位点。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5540-4. doi: 10.1073/pnas.80.18.5540.
8
Identification of the modified nucleotides produced by covalent photoaddition of hydroxymethyltrimethylpsoralen to RNA.鉴定羟甲基三甲基补骨脂素与RNA共价光加成产生的修饰核苷酸。
Nucleic Acids Res. 1981 May 11;9(9):2207-22. doi: 10.1093/nar/9.9.2207.
9
Inactivation of Saccharomyces cells by 8-methoxypsoralen plus pulsed laser irradiation in the wavelength range 308 nm-380 nm.8-甲氧基补骨脂素加308纳米至380纳米波长范围内的脉冲激光辐照对酿酒酵母细胞的灭活作用。
Radiat Environ Biophys. 1986;25(1):55-63. doi: 10.1007/BF01209685.
10
Mutagenesis induced by site specifically placed 4'-hydroxymethyl-4,5',8-trimethylpsoralen adducts.由位点特异性放置的4'-羟甲基-4,5',8-三甲基补骨脂素加合物诱导的诱变作用。
Nucleic Acids Res. 1988 Nov 11;16(21):9961-77. doi: 10.1093/nar/16.21.9961.