染色质纤维尺寸与核小体取向：中子散射研究
Chromatin fiber dimensions and nucleosome orientation: a neutron scattering investigation.
作者信息
Baudy P, Bram S
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3697-714. doi: 10.1093/nar/5.10.3697.
Abstract
Chromatin fibers were studied in solutions of mM monovalent salt by small angle neutron scattering. The variation of the cross section radius of gyration with H2O/D2O contrast shows that DNA is at much larger average radial distances from the fiber axis than histone. Consequently, the coils of DNA in a core particle must be approximately parallel to the fiber direction. The radii of gyration suggest that the maximum diameter of chromatin and nucleosomes is approximately 14 nm and that the DNA id distributed in two radial layers. The concentration dependence of the scattering maxima near 14 nm spacings furnishes independent support for a 14 nm external diameter and can be interpreted by a double DNA layer configuration.
摘要
通过小角中子散射研究了在毫摩尔单价盐溶液中的染色质纤维。回转半径横截面随H2O/D2O对比度的变化表明，DNA距纤维轴的平均径向距离比组蛋白大得多。因此，核心颗粒中DNA的螺旋必须大致平行于纤维方向。回转半径表明染色质和核小体的最大直径约为14nm，并且DNA分布在两个径向层中。14nm间距附近散射最大值的浓度依赖性为14nm的外径提供了独立支持，并且可以通过双DNA层构型来解释。
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