一种新型RNA-RNA交联试剂及其在核糖体5S RNA中的应用。
A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA.
作者信息
Wagner R, Garrett R A
出版信息
Nucleic Acids Res. 1978 Nov;5(11):4065-75. doi: 10.1093/nar/5.11.4065.
Abstract
The synthesis of a new RNA specific bifunctional crosslinking reagent, 1.4-phenyl-diglyoxal, is described which reacts exclusively with guanosines. The properties of the crosslinked products enabled us to develop a straightforward method for identifying the reacted nucleotides. Results obtained with ribosomal 5S RNA of Escherichia coli demonstrate the formation of an intramolecular crosslink between guanosine-2 and guanosine-112 in the stem region.
摘要
本文描述了一种新的RNA特异性双功能交联剂1,4-苯基-二乙二醛的合成，它只与鸟苷发生反应。交联产物的性质使我们能够开发出一种直接的方法来鉴定反应的核苷酸。用大肠杆菌核糖体5S RNA得到的结果表明，在茎区鸟苷-2和鸟苷-112之间形成了分子内交联。
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