College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute of Molecular Medicine, Wuhan University People's Hospital, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, China.
Adv Sci (Weinh). 2023 Apr;10(10):e2206433. doi: 10.1002/advs.202206433. Epub 2023 Feb 3.
Conditional control of RNA structure and function has emerged as an effective toolkit. Here, a strategy based on a one-step introduction of diacylation linkers and azide groups on the 2'-OH of RNA is advance. Selected from eight phosphine reagents, it is found that 2-(diphenylphosphino)ethylamine has excellent performance in reducing azides via a Staudinger reduction to obtain the original RNA. It is demonstrated that the enzymatic activities of Cas13 and Cas9 can be regulated by chemically modified guide RNAs, and further achieved ligand-induced gene editing in living cells by a controllable CRISPR/Cas9 system.
条件控制 RNA 结构和功能已成为一种有效的工具。在这里，提出了一种基于在 RNA 的 2'-OH 上一步引入二酰化连接子和叠氮基团的策略。从八种膦试剂中选择，发现 2-(二苯基膦基)乙胺通过 Staudinger 还原将叠氮化物还原为原始 RNA 的性能优异。结果表明，Cas13 和 Cas9 的酶活性可以通过化学修饰的向导 RNA 进行调节，并通过可控的 CRISPR/Cas9 系统进一步实现活细胞中的配体诱导基因编辑。
