Gurusiddaiah S, Ronald R C
Antimicrob Agents Chemother. 1981 Jan;19(1):153-65. doi: 10.1128/AAC.19.1.153.
Aerobic fermentation of cultures of Cytospora sp. Ehrenb. W.F.P.L. 13A produces a group of novel, broad-spectrum, dilactone, macrocyclic antibiotics. These compounds, named grahamimycins, were isolated from the culture medium by extraction with organic solvents and separated by chromatography. Three active components have been obtained in crystalline form and have been designated grahamimycins A, A1, and B. The most active of these, grahamimycin A, exhibited activity against 36 species of bacteria, 8 species of blue-green algae (cyanobacteria), and 2 species of green algae, and antifungal activity against 5 fungi. Studies on the growth of the Cytospora with respect to formation of antibiotic activity, and the isolation, characterization, and structure elucidation of the grahamimycins are described. These studies indicate that the grahamimycins are distinct from other known antibiotics and represent a new class of antibiotic structures.
壳囊孢属（Cytospora sp. Ehrenb. W.F.P.L. 13A）培养物的需氧发酵产生了一组新型的、广谱的、双内酯大环抗生素。这些化合物被命名为格拉米霉素，通过用有机溶剂萃取从培养基中分离出来，并通过色谱法进行分离。已获得三种活性成分的晶体形式，并将其命名为格拉米霉素A、A1和B。其中活性最强的格拉米霉素A对36种细菌、8种蓝藻（蓝细菌）和2种绿藻具有活性，对5种真菌具有抗真菌活性。本文描述了关于壳囊孢属形成抗生素活性的生长情况，以及格拉米霉素的分离、表征和结构解析。这些研究表明，格拉米霉素与其他已知抗生素不同，代表了一类新的抗生素结构。
