Horváth I, Mocsári E
Arch Virol. 1981;68(2):103-13. doi: 10.1007/BF01314440.
The small intestine of piglets collected during a sudden outbreak of diarrhoeal disease resembling transmissible gastroenteritis (TGE) was examined by light and electron microscopy. The principal histopathological changes were moderate infiltration by mononuclear cells in the lamina propria of the villi and cytoplasmic vacuolation. These were most pronounced in the epithelial cells covering the villous tips. By scanning electron microscopy, the intestinal villi were swollen and the transverse furrows disappeared. Microvilli were reduced in number leaving denuded areas on the brush border of the villous epithelial cells. The ultrastructural changes were restricted to the cytoplasm of affected villous epithelial cells. The cell organelles were missing in rounded areas leaving cleared areas in the cytoplasm. Parallel fascicles and bundles were seen in these areas. Viral particles with an average diameter of 70 nm were found within the dilated apical tubulo-vesicular system, free in the cytoplasm, among the microvilli, or lying free in the intestinal lumen. Viral particles surrounded a non-membrane bound viroplasm in some cases. The negatively stained particles showed a typical coronavirus morphology. These particles were found to be distinct from the known coronaviruses of swine, TGE virus and hemagglutinating encephalomyelitis virus by immune electron microscopy.
对在一场类似于传染性胃肠炎（TGE）的腹泻病突然爆发期间收集的仔猪小肠进行了光镜和电镜检查。主要的组织病理学变化为绒毛固有层单核细胞中度浸润以及细胞质空泡化。这些变化在覆盖绒毛顶端的上皮细胞中最为明显。通过扫描电子显微镜观察，肠绒毛肿胀，横向沟消失。微绒毛数量减少，在绒毛上皮细胞的刷状缘留下裸露区域。超微结构变化局限于受影响的绒毛上皮细胞的细胞质。细胞器在圆形区域缺失，在细胞质中留下清晰区域。在这些区域可见平行的束状和条索状结构。在扩张的顶端管状小泡系统内、细胞质中、微绒毛之间或游离于肠腔内发现了平均直径为70 nm的病毒颗粒。在某些情况下，病毒颗粒围绕着一个无膜结合的病毒质。经负染的颗粒呈现典型的冠状病毒形态。通过免疫电子显微镜发现这些颗粒与已知的猪冠状病毒、TGE病毒和血凝性脑脊髓炎病毒不同。
