Kharchenko E P, Nalivaeva N N, Sokolova T V
Biokhimiia. 1980 Sep;45(9):1630-8.
Using electrophoresis in 12% polyacrylamide gel with 8 M urea and 0.6 mM Triton X-100, the spectra of cationic chromosomal proteins from various tissues and animal species were studied. The tissue specificity of the histone spectra is due to the sex, tissue and specific differences. Histones H2a, H2b, H3 and H4 as well as protamines and protamine-like proteins are represented in chromatin as a sequence of subfractions. The data on heterogeneity of the tissue spectra for the histones can be interpreted in terms of their possible participation in the control of genome functioning.
利用在含有8M尿素和0.6mM Triton X - 100的12%聚丙烯酰胺凝胶中进行电泳，研究了来自各种组织和动物物种的阳离子染色体蛋白的光谱。组蛋白光谱的组织特异性归因于性别、组织和特定差异。组蛋白H2a、H2b、H3和H4以及鱼精蛋白和类鱼精蛋白在染色质中以一系列亚组分的形式存在。关于组蛋白组织光谱异质性的数据可以根据它们可能参与基因组功能控制来解释。
