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细胞的离心淘析(反流离心)

Centrifugal elutriation (counterstreaming centrifugation) of cells.

作者信息

Pretlow T G, Pretlow T P

出版信息

Cell Biophys. 1979 Jun;1(2):195-210. doi: 10.1007/BF02781351.

Abstract

Lindahl first described the separation of cells by velocity sedimentation utilizing a special technique (counterstreaming centrifugation) that was later modified slightly and renamed centrifugal elutriation. Centrifugal elutriation has been applied, with variable degrees of success, to the separation of hemopoietic cells, mouse tumor cells, testicular cells, and a variety of other specialized cells as well as cells in particular phases of the cell cycle. The capacity of the elutriator to separate large numbers of cells is its chief advantage. The purities of the separated cells have not been compared with the purities of cells separated by other methods in most cases; such comparisons would permit more sophisticated comparison of elutriation with other techniques for velocity sedimentation.

摘要

林达尔首次描述了利用一种特殊技术(逆流离心法)通过速度沉降分离细胞,该技术后来略有改进并重新命名为离心淘析法。离心淘析法已被应用于造血细胞、小鼠肿瘤细胞、睾丸细胞以及多种其他特殊细胞的分离,以及细胞周期特定阶段的细胞分离,取得了不同程度的成功。淘析器分离大量细胞的能力是其主要优势。在大多数情况下,尚未将分离细胞的纯度与通过其他方法分离的细胞纯度进行比较;这样的比较将使淘析法与其他速度沉降技术的比较更加精确。

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