哺乳动物细胞中mRNA-蛋白质复合物的表征
Characterization of mRNA-protein complexes from mammalian cells.
作者信息
Sundquist B, Persson T, Lindberg U
出版信息
Nucleic Acids Res. 1977 Apr;4(4):899-915. doi: 10.1093/nar/4.4.899.
Abstract
In a previous report we described the use of oligo(dT)-cellulose for the isolation of mRNA-protein complexes from EDTA-dissociated polysomes extracted from normally growing or adenovirus infected KB-cells (I). Experiments presented here provide evidence that proteins involved in these complexes bind specifically to mRNA since: a) the proteins and mRNA cosediment through sucrose gradients, b) they adsorb and elute from oligo(dT)-cellulose together, and c) analysis of the products from ribonuclease digestion experiments show that the poly (A) end and a separate small fraction of the mRNA are resistant to the enzymes and attached to protein.
摘要
在之前的一份报告中，我们描述了使用寡聚（dT）纤维素从从正常生长或腺病毒感染的KB细胞中提取的经EDTA解离的多聚核糖体中分离mRNA - 蛋白质复合物（I）。此处呈现的实验提供了证据，表明参与这些复合物的蛋白质与mRNA特异性结合，因为：a）蛋白质和mRNA通过蔗糖梯度共同沉降，b）它们一起从寡聚（dT）纤维素上吸附和洗脱，并且c）核糖核酸酶消化实验产物的分析表明，多聚（A）末端和一小部分单独的mRNA对酶具有抗性并与蛋白质相连。
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