Borrebaeck C, Börjeson J
Scand J Clin Lab Invest. 1980 Apr;40(2):169-72. doi: 10.3109/00365518009093021.
The method described is a simple routine assay suited for a short series of serum samples. The time needed for one assay is 2 to 3 min from a stand-by arrangement. The urease is immobilized on controlled pore glass. The beads are placed in the column of an enzyme thermistor unit that is part of a continuous flow system. The heat of reaction when urea is degraded to ammonia and carbon dioxide by immobilized urease is measured and recorded continuously. The technique was investigated as regards to flow dependence, linearity, recovery, precision and some possible interfering substances. The within day precision was 0.8% (C.V.) and the day to day precision, during 56 days, was 3.0% (C.V.). Furthermore, the coefficient of correlation between results obtained with the enzyme thermistor unit and a conventional spectrophotometric method was 0.991.
所述方法是一种适用于少量血清样本的简单常规检测方法。从备用状态开始，一次检测所需时间为2至3分钟。脲酶固定在可控孔径玻璃上。珠子放置在酶热敏电阻单元的柱中，该单元是连续流动系统的一部分。当固定化脲酶将尿素降解为氨和二氧化碳时，反应热被连续测量和记录。对该技术在流量依赖性、线性、回收率、精密度以及一些可能的干扰物质方面进行了研究。日内精密度为0.8%（变异系数），在56天内的日间精密度为3.0%（变异系数）。此外，酶热敏电阻单元获得的结果与传统分光光度法获得的结果之间的相关系数为0.991。
