Bowers L D, Canning L M, Sayers C N, Carr P W
Clin Chem. 1976 Aug;22(8):1314-8.
We used a differential thermal detector in conjunction with an immobilized urease reactor to determine urea in serum. Samples (120 mul) are introduced into a flow stream and passed through an "adiabatic" column, which is packed with enough insolubilized urease to completely convert urea to ammonia and carbon dioxide. Measured temperature changes are directly proportional to the serum urea concentration. Urea in the presence of protein, bilirubin, and hemoglobin can thus be rapidly, simply, and inexpensively measured. Results correlate well with those obtained by the manual diacetyl monoxime and urease/indophenol methods.
我们使用差示热检测器与固定化脲酶反应器相结合来测定血清中的尿素。将样品（120微升）引入流动流中，并通过一个“绝热”柱，该柱填充有足够的不溶性脲酶，以将尿素完全转化为氨和二氧化碳。测得的温度变化与血清尿素浓度成正比。因此，可以快速、简单且廉价地测定存在蛋白质、胆红素和血红蛋白情况下的尿素。结果与通过手动双乙酰一肟法和脲酶/吲哚酚法获得的结果相关性良好。
