Borgens R B, Roederer E, Cohen M J
Science. 1981 Aug 7;213(4508):611-7. doi: 10.1126/science.7256258.
After a weak, steady electric current of approximately 10 microamperes was imposed across the completely severed spinal cord of the larval lamprey Petromyzon marinus, enhanced regeneration was observed in the severed giant reticulospinal neurons. The current was applied with implanted wick electrodes for 5 to 6 days after transection (cathode distal to lesion). The spinal cords were examined 44 to 63 days after the operation by means of intracellular fluorescent dye injections and electrophysiology. Extracellular stimulation of whole cords showed that action potentials in most of the electrically treated preparations were conducted in both directions across the lesion, but they were not conducted in either direction in most of the sham-treated controls. In most of the electrically treated animals, processes from giant axons with swollen irregular tips, indicating active growth, were seen in or across the lesion. Only a few of the sham-treated controls showed these features. It is possible that these facilitated regenerative responses were mediated by the effects of the artificially applied electric fields on the natural steady current of injury entering the spinal lesion.
在对海七鳃鳗幼体（Petromyzon marinus）完全横断的脊髓施加约10微安微弱、稳定的电流后，在横断的巨大网状脊髓神经元中观察到再生增强。在横断后，用植入的灯芯电极施加电流5至6天（阴极位于损伤远端）。术后44至63天，通过细胞内荧光染料注射和电生理学检查脊髓。对整个脊髓的细胞外刺激表明，在大多数经电处理的标本中，动作电位在损伤部位双向传导，但在大多数假处理对照组中，动作电位在任何一个方向都不传导。在大多数经电处理的动物中，在损伤部位或穿过损伤部位可见到来自巨大轴突的、末端肿胀不规则的突起，这表明有活跃生长。只有少数假处理对照组显示出这些特征。这些促进再生的反应可能是由人工施加的电场对进入脊髓损伤部位的自然损伤稳定电流的影响所介导的。
