Schubert R H
Zentralbl Bakteriol Mikrobiol Hyg B. 1981;172(6):528-33.
Studies on the generation time of P. shigelloides and its relation to incubation temperature have shown that this species in marked contrast to A. hydrophila does not multiply in temperature below 8 degrees C. These findings would explain the seasonal variations in the number of isolates from river-water observed in Japan. Colony counts of P. shigelloides organisms on IBG-agar from water of the river Main sampled during the month of August (water temperature 20 degrees C) have shown numbers of 10--100 c.f.u./ml. Thus P. shigelloides is present in surface water in Middle Europe. The presence of the germs could equally be demonstrated in the guts of many specimen of non-preying fish that had been caught during the months of July and August. When determining the bacterial species isolated from such material the possible presence of inositol-positive A. hydrophila strains has to be taken into account. Results of former studies on the suitability of Leifson-agar, Wilson-Blair- agar, SS-agar and IBG-agar then with pure cultures were confirmed by our work with faecal material from fish done this time. While favourable results were obtained on IBG- and SS-agar P. shigelloides could in no case be cultured on Leifson- and Wilson-Blair-agar.
关于类志贺邻单胞菌的代时及其与培养温度的关系的研究表明，与嗜水气单胞菌形成显著对比的是，该菌种在8摄氏度以下的温度中不会繁殖。这些发现可以解释在日本观察到的河水分离株数量的季节性变化。8月份（水温20摄氏度）从美因河采集的水样在IBG琼脂上进行的类志贺邻单胞菌菌落计数显示，数量为10-100 c.f.u./ml。因此，类志贺邻单胞菌存在于中欧的地表水中。在7月和8月捕获的许多非捕食性鱼类的肠道中也同样可以证明这种细菌的存在。在从这类材料中分离细菌种类时，必须考虑到肌醇阳性嗜水气单胞菌菌株可能存在的情况。我们这次对鱼类粪便样本的研究证实了以前关于利夫森琼脂、威尔逊-布莱尔琼脂、SS琼脂和IBG琼脂对纯培养物适用性的研究结果。虽然在IBG琼脂和SS琼脂上获得了良好的结果，但类志贺邻单胞菌在任何情况下都无法在利夫森琼脂和威尔逊-布莱尔琼脂上培养。
