大鼠垂体中的催乳素含量。不同提取方法后的催乳素放射免疫分析。

Prolactin content in rat pituitary gland. RIA of prolactin after different extraction procedures.

作者信息

Haggi E, Aoki A

出版信息

Acta Endocrinol (Copenh). 1981 Jul;97(3):338-42. doi: 10.1530/acta.0.0970338.

DOI:10.1530/acta.0.0970338

Abstract

The prolactin content of rat pituitary varies considerably when determined by RIA, due to incomplete solubilization of prolactin secretory granules with standard procedures for tissue homogenization and centrifugation. Freezing and thawing, Triton X-100 and ultrasonic treatments increased the yield of prolactin significantly but electron microscopy of pellets revealed numerous unmodified secretory granules. Addition of 2.5 M urea produced complete extraction of tissue prolactin confirmed by RIA of supernatants and electron microscopy of pellets.

摘要

当通过放射免疫分析（RIA）测定时，大鼠垂体中的催乳素含量变化很大，这是因为采用标准的组织匀浆和离心程序时，催乳素分泌颗粒的溶解不完全。冻融、Triton X-100和超声处理显著提高了催乳素的产量，但对沉淀进行电子显微镜检查发现有许多未改变的分泌颗粒。添加2.5M尿素可实现组织催乳素的完全提取，这通过对上清液进行放射免疫分析以及对沉淀进行电子显微镜检查得以证实。