Sesma P, Vázquez J J
Am J Anat. 1981 Jun;161(2):247-56. doi: 10.1002/aja.1001610208.
Myelopoiesis in lymph nodes was studied in newborn mice under normal conditions and after treatment with trypan blue and carbon particles. In the mouse lymph node, myelopoiesis normally takes place between the fourth and twelfth day after birth, then decreases while an intense proliferation of the lymphocytic population simultaneously takes place. Myelopoiesis manifests itself as clusters of cells, mainly of the neutrophilic line, located around post-capillary venules (PCV's) in the medulla. The injection of trypan blue or carbon particles (India ink) causes an enhancement of myelopoiesis and a delay in the development of the lymphocytic population. Myelopoiesis includes not only granulopoiesis but also the formation of erythroid clusters and occasional megakaryocytes. Three possibilities should be considered to explain the enhancement of myelopoiesis: 1) blockade of the reticuloendothelial system caused by overloading with particulate matter; 2) induction of humoral myelopoiesis-stimulating factors; and 3) stimulation of pluripotent stem cells through dye injection.
在正常条件下以及在用台盼蓝和碳颗粒处理后，对新生小鼠淋巴结中的髓系造血进行了研究。在小鼠淋巴结中，髓系造血通常在出生后第4天至第12天之间发生，然后减少，与此同时淋巴细胞群体强烈增殖。髓系造血表现为细胞簇，主要是嗜中性粒细胞系，位于髓质中的毛细血管后微静脉（PCV）周围。注射台盼蓝或碳颗粒（印度墨水）会导致髓系造血增强以及淋巴细胞群体发育延迟。髓系造血不仅包括粒细胞生成，还包括红系细胞簇和偶尔的巨核细胞的形成。为了解释髓系造血增强，应考虑三种可能性：1）颗粒物质过载导致网状内皮系统受阻；2）诱导体液性髓系造血刺激因子；3）通过染料注射刺激多能干细胞。
