登革病毒影响伪盾伊蚊细胞中合胞体发育的因素。

Factors affecting syncytial development in Aedes pseudoscutellaris cells by dengue viruses.

作者信息

Kuno G, Moore C G, Sather G E

出版信息

Am J Trop Med Hyg. 1981 Jul;30(4):870-5. doi: 10.4269/ajtmh.1981.30.870.

DOI:10.4269/ajtmh.1981.30.870

Abstract

Several factors that were suspected of affecting the development of syncytia in cultured Aedes pseudoscutellaris cells inoculated with dengue viruses were studied. The results indicated that fresh media (less than 1 week old), low cell density at inoculation (2.8 x 10(5) cells/cm2), and low cell passage level (less than 52 passages) favored the development of syncytia. All three types (1, 2, and 3) of dengue viruses tested could be isolated from human sera by Aedes pseudoscutellaris cell culture by using syncytial development as an indicator, but the isolation rate was lower than that obtained by using intrathoracically inoculated Aedes aegypti mosquitoes.

摘要

对几种被怀疑影响接种登革病毒的伪盾纹伊蚊培养细胞中多核细胞形成的因素进行了研究。结果表明，新鲜培养基（小于1周龄）、接种时的低细胞密度（2.8×10⁵个细胞/cm²）和低细胞传代水平（小于52代）有利于多核细胞的形成。通过以多核细胞形成作为指标，利用伪盾纹伊蚊细胞培养，可从人血清中分离出所有三种测试类型（1型、2型和3型）的登革病毒，但分离率低于通过胸腔内接种埃及伊蚊获得的分离率。