Losev V V, Laznikova T N, Dmitrieva S V
Antibiotiki. 1981 May;26(5):352-7.
The method of differential centrifugation in the sucrose density gradient (SDG) enabled one to trace the changes in the development of the seed and fermentation mycelium of the gentamicin-producing organism. Correlation between gentamicin distribution in the SDG and the culture productivity was found. It was shown that the culture grown under the optimal aeration and agitation conditions was characterized by formation of higher amounts of the mycelium in the 5th and 6th layers of the SDG. Such mycelium was more productive than that from the other SDG layers. The most productive 48-hour seed culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture was used as the seed material, the activity of the fermentation broth was the highest. The method of differential centrifugation in the SDG provides determination of the culture productivity by the volumes of the fermentation mycelium in the 5th and 6th layers or the seed mycelium in the 3rd layer of the SDG.
蔗糖密度梯度（SDG）差速离心法能够追踪庆大霉素产生菌种子和发酵菌丝体发育过程中的变化。发现了SDG中庆大霉素分布与培养物生产力之间的相关性。结果表明，在最佳通气和搅拌条件下培养的培养物，其特征是在SDG的第5层和第6层中形成了更多的菌丝体。这种菌丝体比其他SDG层的菌丝体生产力更高。最具生产力的48小时种子培养物在SDG的第3层中有更显著部分的菌丝体。当这种培养物在SDG的第3层中有更显著部分的菌丝体时。当将这种培养物用作种子材料时，发酵液的活性最高。SDG差速离心法通过SDG第5层和第6层中发酵菌丝体的体积或第3层中种子菌丝体的体积来确定培养物的生产力。
