Losev V V, Kotlenska P V, Tsoneva D P, Laznikova T N
Antibiotiki. 1981 Jul;26(7):496-500.
The development of the seed mycelium at various stages and temperatures was studied with the method of methylene blue decolorization characterizing the respiration level of the culture and its oxidation-reduction (dehydrogenase) activity. Relationship between the time of methylene blue decolorization by the seed mycelium, the quantity of the inoculum transferred from one stage to the other and the temperature of cultivation was shown. The seed mycelium decolorizing methylene blue within 2 minutes was most productive at the biosynthetic stage irrespective of the growth period, biomass volume, cultivation temperature and volume of the inoculum transferred from one stage to the other.
采用亚甲基蓝脱色法研究了种子菌丝体在不同阶段和温度下的发育情况，该方法可表征培养物的呼吸水平及其氧化还原（脱氢酶）活性。研究表明了种子菌丝体使亚甲基蓝脱色的时间、从一个阶段转移到另一个阶段的接种量与培养温度之间的关系。无论生长时期、生物量体积、培养温度以及从一个阶段转移到另一个阶段的接种量如何，在2分钟内使亚甲基蓝脱色的种子菌丝体在生物合成阶段最具生产力。
