用外部四乙铵离子抑制黏液虫巨大轴突中的钾离子电导。
Inhibition of potassium conductance with external tetraethylammonium ion in Myxicola giant axons.
作者信息
Wong B S, Binstock L
出版信息
Biophys J. 1980 Dec;32(3):1037-42. doi: 10.1016/S0006-3495(80)85034-X.
Abstract
In voltage clamp experiments, externally applied tetraethylammonium ion (TEA) was found to have minimal effects on transient sodium currents and to suppress steady-state potassium currents of Myxicola giant axons by causing a specific decrease in the maximum potassium conductance gK. The dose-response curve suggests a one-to-one stoichiometry for TEA-receptor binding with an apparent dissociation constant on 24 mM. The suppression of IK is essentially reversible. Experiments performed on high external potassium ion concentrations indicate that both outward and inward IK were blocked by external TEA. The results thus suggest the presence of TEA receptors on the outer surface of Myxicola axonal membrane similar to those reported in the frog node.
摘要
在电压钳实验中，发现外部施加的四乙铵离子（TEA）对瞬时钠电流影响极小，并且通过使最大钾电导gK特异性降低来抑制黏液虫巨轴突的稳态钾电流。剂量反应曲线表明TEA与受体结合存在一对一的化学计量关系，其表观解离常数为24 mM。IK的抑制作用基本是可逆的。在高外部钾离子浓度下进行的实验表明，外部TEA可阻断外向和内向IK。因此，结果表明黏液虫轴突膜外表面存在与青蛙节点中报道的类似的TEA受体。
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