Andersson T R, Svennevig J L
Clin Exp Immunol. 1981 Apr;44(1):167-72.
Mononuclear cells were isolated from peripheral blood and analysed with T and B markers (E rosettes and SIg) and on contaminating monocytes and PMN. The suspensions contained 63.3 +/- 4.8% T lymphocytes, 11.4 +/- 3.8% B lymphocytes, 9.0 +/- 5.5% null lymphocytes, 15.1 +/- 3.8% monocytes and 1.1 +/- 0.6% PMN. Of all cells, 27.6 +/- 12.1% formed EA rosettes with OR1R2 red cells coated with anti-CD Ripley. In preparations fixed after cytocentrifugation, the EA rosette-forming cells were studied with regard to esterase activity. The proportion of cells with detectable Fc receptors was further studied in purified T lymphocyte and in monocyte suspensions. Finally, EA rosettes were isolated by gradient centrifugation and the rosette forming cells studied with conventional T and B markers. All procedures gave corresponding results: on average 11-14% of the T lymphocytes and nearly 100% of the null cells formed EA rosettes, while only 2% of the B lymphocytes had detectable Fc receptors. Of the purified monocyte and PMN populations, on average 72 +/- 10.5 and 14.5 +/- 5.4%, respectively, formed EA rosettes. Thus, the Ripley assay, representing an important marker for null lymphocytes, cannot be regarded as specific for this population of white blood cells.
从外周血中分离出单核细胞，并用T和B标记物（E花环和表面免疫球蛋白）以及对污染的单核细胞和多形核白细胞进行分析。悬浮液中含有63.3±4.8%的T淋巴细胞、11.4±3.8%的B淋巴细胞、9.0±5.5%的裸淋巴细胞、15.1±3.8%的单核细胞和1.1±0.6%的多形核白细胞。在所有细胞中，27.6±12.1%的细胞与用抗CD Ripley包被的OR1R2红细胞形成EA花环。在细胞离心涂片固定后的制剂中，对EA花环形成细胞的酯酶活性进行了研究。在纯化的T淋巴细胞和单核细胞悬浮液中进一步研究了具有可检测Fc受体的细胞比例。最后，通过梯度离心分离EA花环，并对花环形成细胞用传统的T和B标记物进行研究。所有程序都得到了相应的结果：平均11 - 14%的T淋巴细胞和近100%的裸细胞形成EA花环，而只有2%的B淋巴细胞具有可检测的Fc受体。在纯化的单核细胞和多形核白细胞群体中，分别平均有72±10.5%和14.5±5.4%形成EA花环。因此，Ripley检测法作为裸淋巴细胞的一个重要标记物，不能被认为对这一白细胞群体具有特异性。
