Mueller J, Brun del Re G, Buerki H, Keller H U, Hess M W, Cottier H
Eur J Immunol. 1975 Apr;5(4):270-4. doi: 10.1002/eji.1830050411.
A modified technique was used to demonstrate lymphocytic acid alpha-naphthyl acetate esterase activity in frozen sections of mouse lymphoid tissue or smears. Positive, dot-like reaction products were noticed in more than 94% of the lymphocytes located in the diffuse cortical ("paracortical", "thymus-dependent") area of mesenteric lymph nodes of young adult ICR mice. Almost identical values were found for lymphocytes in the cisterna chyli. In contrast, the follicular cortex which is predominantly occupied by B cells, contained less than 7% esterase-positive lymphocytes. In vitro, cytotoxic anti-theta serum destroyed the vast majority of esterase positive lymphocytes while esterase-negative lymphocytes were resistent to this treatment. In mesenteric nodes of nude BALB/c (nu/nu) mice, follicular cortex and paracortex together contained approximately 16 times less esterase-positive lymphocytes than in the immunologically competent hybrid BALB/c (nu/+) animals. These findings indicate that non-specific acid esterase activity may serve as a criterion to differentiate peripheral T and B lymphocytes in lymph node sections and smears of mice by light microscopy. Possible implications of this enzyme activity in thymus-derived lymphocytes are discussed.
采用改良技术在小鼠淋巴组织冰冻切片或涂片上显示淋巴细胞酸性α-萘乙酸酯酶活性。在成年ICR小鼠肠系膜淋巴结弥漫性皮质（“副皮质”，“胸腺依赖”）区域的淋巴细胞中，超过94%的细胞出现阳性点状反应产物。乳糜池中淋巴细胞的情况几乎相同。相比之下，主要由B细胞占据的滤泡皮质中，酯酶阳性淋巴细胞不到7%。在体外，细胞毒性抗θ血清可破坏绝大多数酯酶阳性淋巴细胞，而酯酶阴性淋巴细胞对此处理有抗性。在裸BALB/c（nu/nu）小鼠的肠系膜淋巴结中，滤泡皮质和副皮质中酯酶阳性淋巴细胞的数量比具有免疫活性的杂种BALB/c（nu/+）动物少约16倍。这些发现表明，非特异性酸性酯酶活性可作为通过光学显微镜在小鼠淋巴结切片和涂片中区分外周T和B淋巴细胞的标准。文中还讨论了这种酶活性在胸腺来源淋巴细胞中的可能意义。
