Selden S C, Rabinovitch P S, Schwartz S M
J Cell Physiol. 1981 Aug;108(2):195-211. doi: 10.1002/jcp.1041080210.
Colchicine and vinblastine inhibited endothelial cell migration but had no effect on the stimulation of replication seen at wound edges in cultures of endothelium at stationary density. This is in contrast to the effects of cytochalasins which inhibit both migration and replication at wound edges. Moreover, colchicine and vinblastine stimulated cell replication in the unwounded, confluent monolayer. This effect has kinetics similar to the stimulation of replication at a wound edge and is associated with an initial retraction of cell borders, leaving gaps between cells. Cytochalasin D inhibited the growth response to microtubule disrupting agents but did not prevent cell retraction. Stimulation of replication by microtubule disrupting agents was not dependent on serum but was synergistic with serum in cultures rinsed repeatedly with serum-free medium. The replication occurred prior to any cell loss. When, however, cells were allowed to complete mitosis, about one-half of the daughter cells detached from the monolayer so that there was no increase in cell density. We conclude that microtubule disrupting agents are the first agents found to be effective in stimulating growth of vascular endothelium at saturation density. These data further suggest that colchicine and vinblastine stimulate cell growth in a manner similar to wounding, where cell movement is a prerequisite to cell replication.
秋水仙碱和长春花碱抑制内皮细胞迁移，但对处于静止密度的内皮细胞培养物伤口边缘处出现的复制刺激没有影响。这与细胞松弛素的作用相反，细胞松弛素会抑制伤口边缘处的迁移和复制。此外，秋水仙碱和长春花碱会刺激未受伤的汇合单层细胞的复制。这种效应的动力学与伤口边缘处复制刺激的动力学相似，并且与细胞边界的初始回缩有关，细胞之间会出现间隙。细胞松弛素D抑制了对微管破坏剂的生长反应，但并未阻止细胞回缩。微管破坏剂对复制的刺激不依赖于血清，但在无血清培养基中反复冲洗的培养物中，与血清具有协同作用。复制发生在任何细胞损失之前。然而，当细胞完成有丝分裂时，约一半的子细胞从单层中脱离，因此细胞密度没有增加。我们得出结论，微管破坏剂是首批被发现对饱和密度下的血管内皮细胞生长有刺激作用的药物。这些数据进一步表明，秋水仙碱和长春花碱以类似于伤口的方式刺激细胞生长，在伤口处细胞运动是细胞复制的先决条件。
