Hunter J T, Ashley M P, Sukumar S, Sugimoto T, Zbar B, Rapp H J, Yarkoni E
J Exp Med. 1981 Aug 1;154(2):253-61. doi: 10.1084/jem.154.2.253.
The malignant disease produced in guinea pigs by intradermal inoculation of line-10 was allowed to progress to stage II, at which time the dermal tumor and the first draining lymph node were grossly evident. At that stage, the external appearance of the next draining lymph node was normal, but it contained tumor cells. Limited surgery consisting of excision of the dermal tumor and first draining lymph node was not curative; palpable metastases developed in the second and other draining lymph nodes, and at autopsy, some animals were found to have gross, visible lung metastases. Immunization of guinea pigs with a mixture of irradiated syngeneic tumor cells plus mycobacterial cell walls in an oil-in-water emulsion eradicated tumor cells remaining in lymph nodes after limited surgery for stage II experimental cancer and prevented progression of the disease to stage III. Tumor intravenously implanted in the lungs of animals after limited surgery for stage II disease was also eliminated by immunization.
通过皮内接种10号线在豚鼠身上产生的恶性疾病发展到II期，此时皮肤肿瘤和第一个引流淋巴结肉眼可见。在那个阶段，下一个引流淋巴结的外观正常，但含有肿瘤细胞。仅切除皮肤肿瘤和第一个引流淋巴结的有限手术无法治愈；在第二个和其他引流淋巴结中出现了可触及的转移灶，尸检时发现一些动物有明显的肺转移灶。用辐照后的同基因肿瘤细胞与分枝杆菌细胞壁的混合物在水包油乳剂中对豚鼠进行免疫，可根除II期实验性癌症有限手术后淋巴结中残留的肿瘤细胞，并防止疾病进展到III期。对II期疾病进行有限手术后静脉植入动物肺部的肿瘤也可通过免疫消除。
