Sukumar S, Hunter J T, Terata N, Rapp H J
Cancer Immunol Immunother. 1983;14(3):151-4. doi: 10.1007/BF00205353.
Strain-2 guinea pigs, each with microscopic deposits of line 10 hepatocarcinoma in the liver, were treated by ID immunization with a mixture of irradiated tumor cells and an oil-in-water emulsion containing cell walls of Mycobacterium bovis strain Bacillus Calmette-Guérin (BCG CWE). Injection of line 10 hepatoma cells into the hepatic portal vein led to the development of tumor foci in the liver, metastasis in the hepatic lymph node, malignant ascites, and death. Active immunization using irradiated line 10 cells and BCG CWE was effective therapy when administered 1, 7, and 14 days after intraportal injection of line 10 cells. Effective immunization required both irradiated line 10 tumor cells and the BCG cell wall emulsion. Immunization with BCG CWE admixed with irradiated line 1 tumor cells, a hepatoma antigenically distinct from line 10, did not prevent outgrowth of line 10 deposits in the liver. Animals rendered free of disease could reject a challenge of line 10 tumor cells but not of line 1 tumor cells.
2号品系豚鼠，每只在肝脏中有10号线肝癌的微小沉积物，通过皮内免疫接种经辐照的肿瘤细胞与含有卡介苗菌株（BCG CWE）细胞壁的水包油乳剂的混合物进行治疗。将10号线肝癌细胞注入肝门静脉会导致肝脏中出现肿瘤病灶、肝淋巴结转移、恶性腹水并导致死亡。在门静脉内注射10号线细胞后的第1、7和14天给予经辐照的10号线细胞和BCG CWE进行主动免疫是有效的治疗方法。有效的免疫需要经辐照的10号线肿瘤细胞和BCG细胞壁乳剂两者。用与经辐照的1号线肿瘤细胞混合的BCG CWE进行免疫，1号线肿瘤细胞是一种抗原性与10号线不同的肝癌，不能阻止肝脏中10号线沉积物的生长。无病的动物可以排斥10号线肿瘤细胞的攻击，但不能排斥1号线肿瘤细胞的攻击。
