Toda T, Leszczynski D, Kummerow F
J Pathol. 1981 Jul;134(3):219-31. doi: 10.1002/path.1711340306.
Five-day-old female chicks were fed diets containing testosterone, estradiol, and cholesterol for 2 months. Cholesterol supplementation of 1 per cent. resulted in production of lipid vacuoles in the interlamellar connective tissue cells and to a lesser extent in the smooth muscle cells in the aorta; cholesterol had no effect on the coronary artery. The vasculo-toxic nature of estrogen was indicated when 0.05 per cent. dietary estradiol produced more accumulations of lipid vacuoles in smooth muscle cells, more extracellular lipid, and more smooth muscle cell death than cholesterol feeding. A dietary combination of cholesterol plus estradiol produced severe lipid deposition throughout the entire thickness of ascending aorta, and degenerative atherosclerotic changes in the coronary artery. Combined testosterone and estradiol supplementation resulted in peculiar dwarf chickens which exhibited increased vascular smooth muscle cell mitosis and degeneration.
给5日龄的雌性雏鸡喂食含睾酮、雌二醇和胆固醇的日粮，持续2个月。补充1%的胆固醇会导致主动脉层间结缔组织细胞以及程度较轻的平滑肌细胞中出现脂质空泡；胆固醇对冠状动脉没有影响。当日粮中含有0.05%的雌二醇时，雌激素的血管毒性就表现出来了，与喂食胆固醇相比，它会使平滑肌细胞中脂质空泡的积累更多、细胞外脂质更多，且平滑肌细胞死亡更多。胆固醇加雌二醇的日粮组合会导致升主动脉全层出现严重的脂质沉积，以及冠状动脉发生退行性动脉粥样硬化改变。同时补充睾酮和雌二醇会导致雏鸡出现奇特的侏儒症，表现为血管平滑肌细胞有丝分裂增加和细胞变性。
