Kasschau M R, Meyn R E
J Toxicol Environ Health. 1981 Jan;7(1):9-18. doi: 10.1080/15287398109529954.
The effects of chronic treatments with HgCl2 on cell survival, DNA replication, and cell progression in cultured Chinese hamster ovary cells were investigated. The ability of these cells to recover from the effects was also characterized. Exposure of cells to 4 X 10(-5) M HgCl2 for 30 min killed about 50% of the cells, and this proportion did not increase with continued exposure up to 24 h. The rate of DNA replication was reduced to 28% of the control rate in the presence of HgCl2. However, when the cells were returned to medium without HgCl2, the rate of DNA replication recovered to 88% of control after 3 h of exposure and 55% of control after 8 h of exposure. The cell doubling time was increased from a control value of 16 h to 31 h in the presence of HgCl2. When the exposed cells were returned to medium without HgCl2, the doubling time returned to 16 h. The rate of progression of cells from G1 phase to S phase was greatly reduced in the presence of HgCl2, and no recovery was observed in this case when the cells were transferred to normal medium. These findings suggested a correlation between ability to recover and the cytotoxic effects of HgCl2.
研究了用氯化汞长期处理对培养的中国仓鼠卵巢细胞的细胞存活、DNA复制和细胞进程的影响。还对这些细胞从这些影响中恢复的能力进行了表征。将细胞暴露于4×10（-5）M氯化汞中30分钟可杀死约50%的细胞，并且随着持续暴露至24小时，这一比例并未增加。在氯化汞存在的情况下，DNA复制速率降至对照速率的28%。然而，当细胞回到不含氯化汞的培养基中时，暴露3小时后DNA复制速率恢复至对照的88%，暴露8小时后恢复至对照的55%。在氯化汞存在的情况下，细胞倍增时间从对照值16小时增加到31小时。当暴露的细胞回到不含氯化汞的培养基中时，倍增时间恢复到16小时。在氯化汞存在的情况下，细胞从G1期进入S期的进程速率大大降低，并且在这种情况下，当细胞转移到正常培养基中时未观察到恢复。这些发现表明恢复能力与氯化汞的细胞毒性作用之间存在相关性。
