采用气相色谱法测定兔体内安替比林：一种研究影响药物氧化代谢因素的可靠方法。

Antipyrine estimations in the rabbit using gas--liquid chromatography: a reliable method for studying factors affecting oxidative drug metabolism.

作者信息

Chambers D M, Jefferson G C

出版信息

J Pharmacol Methods. 1981 Jan;5(1):59-66. doi: 10.1016/0160-5402(81)90103-0.

Abstract

The application of a gas--liquid chromatographic (GLC) method to measurements of antipyrine in plasma for the estimation of antipyrine half-life (T1/2) in the rabbit is described and is compared with previous GLC methods. It has been established that the estimation of antipyrine can be achieved with acceptable precision and that rabbits can act as their own controls in the study of factors that might alter T1/2. Iproniazid phosphate, 50 mg/kg IP, was shown to produce a mean increase of 170% in T1/2.

摘要

本文描述了一种气液色谱（GLC）方法在测量兔血浆中安替比林以估算其半衰期（T1/2）方面的应用，并与以往的GLC方法进行了比较。已证实，安替比林的估算可达到可接受的精度，并且在研究可能改变T1/2的因素时，兔子可作为自身对照。结果显示，腹腔注射50mg/kg磷酸异烟肼可使T1/2平均增加170%。