Armon C, Kerem D
Undersea Biomed Res. 1981 Jun;8(2):121-6.
Combinations of thiopentone, light mineral oil, and pressure to as much as 160 ATA were applied to the isolated, thermostated (18 degrees C) frog sciatic nerve. Amplitude and rise rate of the compound action potential were examined and compared to the response in Ringer's solution. Immersion in mineral oil caused no change in the response. Pressure, whether applied by helium or after immersion in mineral oil, caused mainly reversible slowing of the response, as did thiopentone by itself, up to at least 5.1 mM. Thiopentone (3.2mM) and helium pressure up to 114 ATA were reversibly synergistic, in that the response was markedly slowed and reduced. Nerves that had been immersed in 3.2 mM thiopentone were placed in mineral oil; this procedure caused a reduction in the size of the response. Application of pressure then eliminated the response, and decompression did not lead to its recovery until the nerve was repeatedly rinsed in Ringer's solution. The findings question the inertness of mineral oil to excitable membranes and indicate that caution should be exercised in its use as a compression medium.
将硫喷妥钠、轻质矿物油以及高达160个绝对大气压的压力作用于分离的、恒温（18摄氏度）的青蛙坐骨神经。检测复合动作电位的幅度和上升速率，并与在林格氏液中的反应进行比较。浸入矿物油对反应无影响。无论是通过氦气施加压力还是在浸入矿物油后施加压力，主要都会导致反应出现可逆性减慢，硫喷妥钠单独作用时也是如此，至少在浓度达到5.1毫摩尔时是这样。3.2毫摩尔的硫喷妥钠和高达114个绝对大气压的氦气压力具有可逆性协同作用，即反应明显减慢且幅度减小。将浸入3.2毫摩尔硫喷妥钠中的神经置于矿物油中；这一操作会导致反应幅度减小。然后施加压力会使反应消失，减压后反应直到神经在林格氏液中反复冲洗后才恢复。这些发现对矿物油对可兴奋膜的惰性提出了质疑，并表明在将其用作压缩介质时应谨慎。
