Hale D C, Thrupp L D, Matsen J M
Am J Clin Pathol. 1981 Aug;76(2):208-11. doi: 10.1093/ajcp/76.2.208.
Urine screening for bacteriuria by light-scatter photometry (Autobac) was evaluated for accuracy and compared with a colony count by the calibrated loop method. Incubation time, inoculum size, precision, and interference of particulate matter were evaluated in an effort to standardize the screening procedure. Results showed that urines could be accurately screened for Enterobacteriaceae by inoculating a single Autobac cuvette chamber with 0.1 or 0.2 ml of urine and determining the voltage change after four hours. A change of greater than or equal to 0.2 units indicates significant bacteriuria. Decreased accuracy was noted for urines having greater than 10(5) cfu/ml of Pseudomonas species or gram-positive cocci, possibly because these organisms grow more slowly.
通过光散射光度法（自动细菌计数仪）对尿液进行菌尿筛查，评估其准确性，并与校准环法的菌落计数进行比较。为了使筛查程序标准化，对孵育时间、接种量、精密度和颗粒物干扰进行了评估。结果表明，通过向单个自动细菌计数仪比色皿腔室接种0.1或0.2毫升尿液，并在4小时后测定电压变化，可以准确筛查出尿液中的肠杆菌科细菌。电压变化大于或等于0.2个单位表明存在显著菌尿。对于假单胞菌属或革兰氏阳性球菌菌落形成单位/毫升大于10⁵的尿液，准确性降低，可能是因为这些微生物生长较慢。
