Kolbeck J C, Padgett R A, Estevez E G, Harrell L J
J Clin Microbiol. 1985 Apr;21(4):527-30. doi: 10.1128/jcm.21.4.527-530.1985.
A rapid screening test (45 min) for bacteriuria was evaluated in 1,000 clinical urine specimens. The test procedure is based upon firefly luciferase analysis of bacterial ATP and uses the Lumac kit and Lumac M2010 Biocounter (3M Co., St. Paul, Minn.). The procedure allows for removal and destruction of nonbacterial ATP and subsequent analysis of bacterial ATP by firefly luciferase with a single photon counter. Results, expressed in relative light units, were compared with actual CFU by the calibrated loop technique. Sensitivities and specificities were calculated separately for clean-catch midstream specimens and for urines obtained by catheterization. The sensitivity for 719 clean-catch specimens with a Lumac cutoff of greater than or equal to 500 relative light units, representing greater than or equal to 10(5) CFU/ml, was 93%. The sensitivity for 281 catheterized specimens with a Lumac cutoff of greater than or equal to 200 relative light units, representing greater than or equal to 10(4) CFU/ml, was 95%. There were 19 false-negative results in the 1,000 specimens tested; more than 50% of these were contaminated cultures and were not considered significant in determining bacteriuria. In conclusion, the Lumac bioluminescence assay is a reliable, rapid bacteriuria screening technique with the potential of reducing the laboratory cost and for reducing the turnaround time in processing negative urine cultures.
我们对一种用于检测菌尿症的快速筛查试验（45分钟）在1000份临床尿液标本中进行了评估。该试验程序基于萤火虫荧光素酶对细菌ATP的分析，并使用Lumac试剂盒和Lumac M2010生物计数器（明尼苏达州圣保罗3M公司）。该程序能够去除和破坏非细菌ATP，随后通过单光子计数器利用萤火虫荧光素酶对细菌ATP进行分析。以相对光单位表示的结果通过校准环技术与实际菌落形成单位进行比较。分别计算了清洁中段尿标本和导尿获取尿液的敏感性和特异性。对于719份清洁中段尿标本，当Lumac临界值大于或等于500相对光单位（相当于大于或等于10⁵CFU/ml）时，敏感性为93%。对于281份导尿标本，当Lumac临界值大于或等于200相对光单位（相当于大于或等于10⁴CFU/ml）时，敏感性为95%。在检测的1000份标本中有19个假阴性结果；其中超过50%是污染培养物，在确定菌尿症时不被视为有意义。总之，Lumac生物发光测定法是一种可靠、快速的菌尿症筛查技术，具有降低实验室成本和缩短阴性尿液培养处理周转时间的潜力。
