Olszewski W, Darzynkiewicz Z, Rosen P P, Schwartz M K, Melamed M R
Cancer. 1981 Aug 15;48(4):985-8. doi: 10.1002/1097-0142(19810815)48:4<985::aid-cncr2820480422>3.0.co;2-q.
Flow cytometry analysis of cell cycle distribution was carried out on acridine-orange-stained cell suspensions freshly prepared from carcinoma of the breast of 90 women, and compared with control, benign breast specimens from 10 women. There was a significantly greater proportion of cells in S (9.1% +/- 5.8) and G2 + M (8.0 +/- 6.0) phases of the cycle in breast cancer specimens than in the S (1.5% +/- 0.6) and G2 + M (2.5 +/- 0.9) phases of the cycle in benign, control specimens of breast tissue. Great variation was noted among the breast cancers, with medullary carcinomas having the highest percentage of cells in S + G2 + M, and papillary, tubular, colloid and well-differentiated duct carcinomas having the lowest. Tumors from premenopausal women classified as estrogen receptor negative had significantly more cells in S + G2 + M than did tumors from postmenopausal women that were estrogen-receptor positive.
对从90名女性乳腺癌患者新鲜制备的吖啶橙染色细胞悬液进行细胞周期分布的流式细胞术分析，并与10名女性的对照良性乳腺标本进行比较。乳腺癌标本中处于细胞周期S期（9.1%±5.8）和G2+M期（8.0±6.0）的细胞比例明显高于良性对照乳腺组织标本中处于细胞周期S期（1.5%±0.6）和G2+M期（2.5±0.9）的细胞比例。乳腺癌之间存在很大差异，髓样癌中处于S+G2+M期的细胞百分比最高，而乳头状癌、管状癌、胶样癌和高分化导管癌中该比例最低。绝经前女性雌激素受体阴性的肿瘤中处于S+G2+M期的细胞明显多于绝经后女性雌激素受体阳性的肿瘤。
