Takahashi M, Arai H, Kokubo T, Furukawa F, Kurata Y, Ito N
Gan. 1980 Dec;71(6):825-31.
Transmission electron microscopic studies of precancerous and cancerous lesions in the pancreas of hamsters induced by N-nitrosobis(2-oxopropyl)amine (BOP) are presented. BOP was injected subcutaneously once weekly for 10 weeks and hamsters were sacrificed every 5 weeks after initiation of the experiment. The ultrastructural findings indicated that serial changes occurred in the epithelium of the pancreatic duct. The epithelial cells became cuboidal and showed increased secretions at 5 weeks. Probably precancerous cells with prominent nucleoli and irregular rough endoplasmic reticulum were found in the main duct at 10 weeks. At 15 weeks, pancreatic tumors forming a duct arrangement were seen, in good accord with the histological appearance. Well differentiated adenocarcinoma cells showing a tubular pattern had oval nuclei with granular chromatin. Poorly developed rough endoplasmic reticulum was irregularly distributed throughout the cytoplasm and the cell surface was covered with microvilli. Poorly differentiated adenocarcinoma showed poor gland formation and had distorted nuclei with prominent nucleoli. These cells were loosely joined. Mitochondria and rough endoplasmic reticulum were poorly developed, and the tumor cells were devoid of secretory granules. The most characteristic and common change of the precancerous and cancerous lesions in this experiment was the appearance of numerous microvilli on the luminal surface and loss of cytodifferentiation. These findings were obviously different from those of normal epithelial cells or those seen in inflammation. The findings in this study confirm that the pancreatic carcinoma induced by N-nitrosobis(2-oxopropyl)amine in Syrian hamsters is of duct cell origin. No evidence of acinar cells was obtained.
本文呈现了用N-亚硝基双(2-氧代丙基)胺（BOP）诱导的仓鼠胰腺癌前病变和癌性病变的透射电子显微镜研究结果。每周皮下注射一次BOP，持续10周，实验开始后每5周处死一批仓鼠。超微结构研究结果表明，胰腺导管上皮发生了一系列变化。在第5周时，上皮细胞变为立方形，分泌物增多。在第10周时，在主导管中发现了可能具有明显核仁及不规则粗面内质网的癌前细胞。在第15周时，可见形成导管排列的胰腺肿瘤，与组织学表现相符。高分化腺癌细胞呈管状排列，核呈椭圆形，染色质呈颗粒状。发育不良的粗面内质网不规则地分布于整个细胞质中，细胞表面覆盖有微绒毛。低分化腺癌腺管形成不良，细胞核扭曲，核仁突出。这些细胞连接松散。线粒体和粗面内质网发育不良，肿瘤细胞无分泌颗粒。本实验中癌前病变和癌性病变最典型和常见的变化是管腔表面出现大量微绒毛及细胞分化丧失。这些发现明显不同于正常上皮细胞或炎症所见。本研究结果证实，叙利亚仓鼠中由N-亚硝基双(2-氧代丙基)胺诱导的胰腺癌起源于导管细胞。未发现腺泡细胞的证据。
