Masuda N, Ellen R P, Grove D A
J Bacteriol. 1981 Sep;147(3):1095-104. doi: 10.1128/jb.147.3.1095-1104.1981.
Fibrils of Actinomyces viscosus WVU627 (numerical taxonomy cluster 1) were obtained by homogenization and purified by ultrafiltration, ammonium sulfate precipitations, gel filtration, and ion-exchange chromatography. Electron microscopy and resolution of a single band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis attested to the purity of the preparation. Purified fibrils were composed mainly of protein; small quantities of carbohydrate and phosphorus were detected. Immunoelectrophoresis revealed only a single precipitable antigen, which migrated slightly toward the anode, in reactions between purified fibrils and antiserum raised against either whole bacterial cells or the purified fibrils themselves. Immunoelectron microscopy with ferritin-conjugated antifibril antibody hemagglutination inhibition, and bacterial agglutination tests demonstrated that fibrils of Actinomyces viscosus cluster 1 strains shared some common antigens with clusters 2, 3, 4 and 6, but did not cross-react with typical Actinomyces naeslundii of cluster 5. Stability tests revealed that after heat or alkali treatment, the fibrils lost their antigenicity and disappeared from electron micrographs. They were affected less by sodium dodecyl sulfate, sonic, or acid treatments.
粘性放线菌WVU627（数值分类簇1）的纤丝通过匀浆获得，并经超滤、硫酸铵沉淀、凝胶过滤和离子交换色谱法纯化。电子显微镜检查以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中单一条带的分辨率证明了制剂的纯度。纯化的纤丝主要由蛋白质组成；检测到少量的碳水化合物和磷。免疫电泳显示，在纯化的纤丝与针对全菌细胞或纯化纤丝本身产生的抗血清的反应中，只有一种可沉淀抗原，该抗原略微向阳极迁移。用铁蛋白偶联的抗纤丝抗体进行免疫电子显微镜检查、血凝抑制试验和细菌凝集试验表明，粘性放线菌簇1菌株的纤丝与簇2、3、4和6有一些共同抗原，但与簇5的典型内氏放线菌无交叉反应。稳定性试验表明，经过加热或碱处理后，纤丝失去抗原性并从电子显微镜照片中消失。它们受十二烷基硫酸钠、超声或酸处理的影响较小。
